Subject Index
A
A system (non-exchanging carrier), 81-82 Absolute inhibitors, 282
Absorption, see specific compounds Acetabularia, morphogenic changes in
195
Acetohydroxamic acid, 190 Acetylaranotin, 258
Acetylcholine, effect on K-inhibited amino acid transport, 87 iV-Acetylhydroxyurea, 190 iV-Acetylimidazole, effect on NaK-
ATPase, 34
iV-Acetylphenylalanyl-tRNA, 336 Acetyl phosphate, 4
Acid phosphatase, isozymes of, 397 Acridine-induced frame-shift mutations,
229
Acridine orange, 182 Acridines, 226, 228, 229
intercalation with DNA, 176 Acriflavine, DNA polymerase and, 195 ACTH, see also Adrenocorticotropin
cyclic AMP and, 367 lipolytic activity of, 375
melanocyte-stimulating activity of, 375 Actidione, 258
Actinobolin, 342 Actinomycin(s), 111
binding to DNA, 176, 323-325 chemistry of, 177
immunosuppression and, 113 mechanism of action, 177
Actinomycin C, renal homograft and, 126
Actinomycin D, 285, 287, 290 AIB uptake and, 95
amino acid transport and, 88 chromosome puffing and, 161 cycloleucine and, 96
deoxyguanosine residues and, 250 DNA-dependent RNA synthesis and,
250, 291 ITSC and, 245
RNA polymerase and, 160 RNA synthesis and, 120
RNA tumor viruses and, 251-252 Rous sarcoma virus and, 197 virus-specific RNA synthesis and,
250-257
Active center of erythrocyte K-depen- dent phosphatase, 14
Active transport, 50 Acyl-D-alanyl-D-alanine, 310
1-Adamantanamine hydrochloride, 238, 239, see also Amantadine
Adaptation temperature and amino acid accumulation, 98-99
Adaptive enzymes and glucose effect, 142
Adenine
binding to nogalamycin, 187 deamination by nitrous acid, 224 Adenohypophyseal hormones, 367 Adenovirus, 262
ITSC and, 246
rifampicin and, 247, 249 Adenyl cyclase, 364, 367 ADH, see Alcohol dehydrogenase Adrenal corticoids, 111
immunosuppression and, 113 inflammatory response, 123 toxicity of, 129
Adrenal steroids and amino acid trans- port, 96-97
Adrenalectomized rats amino acid levels in, 100 glycine transport in, 96-97 insulin in, 95
Adrenocortical steroids and amino acid transport, 96-97
470
SUBJECT I N D E X 471
Adrenocorticotropin, 362-365, 366 structure-activity relationships,
373-377
Aerobacter aerogenes
derepression of histidine synthesis in, 149
transient repression in, 144
Aerobic tissues, LDH isozymes in, 401 Affinity constant, effect of sodium ion,
84
Aflatoxin (s), 186-187
binding to single-stranded DNA, 187 Aflatoxin B,, 187
tryptophan pyrrolase induction and, 187
tyrosine transaminase induction and, 187
African green monkey kidney cells, 255 AGMK cells, see African green monkey
kidney cells
AIB, 92, see also α-Aminoisobutyric acid accumulation of, 85, 97
temperature dependence of membrane permeability to, 99
transport
estradiol and, 95
follicle-stimulating hormone and, 95-96
glucagon and, 95 thyroid hormones and, 96 uptake
antibiotics, effect of, 95 calcium and, 88 epinephrine and, 94 Alamethicin, 319 Alanine, 74
polymeric glutamic dehydrogenase and, 396
transport
anaerobic stimulation of, 98 calcium and, 88
kinetic model of, 83 sodium ion and, 83 /3-Alanine, 76
transport, 98
effect of sodium ion, 84 D-Alanine carboxypeptidase, 309 D-Alanyl-D-alanine, 309
9jS-Alanylfysine vasopressin, 372
Albocycline, 342
Alcohol dehydrogenase, zymogram pat
tern of, 389-390
Alcohols, effect on NaK-ATPase, 34 Aldolase, 385
subunit interaction and, 394 Aldolase isozymes
classification of, 385 function of, 385 Aldosterone
sodium transport and, 160 transcription and, 160 Aldrin, 33
Alfalfa mosaic virus, 287 8-azaguanine and, 297
Alkali metal ions and monamycin, 313 Alkaline phosphatase
isozymes of, 397 positive control and, 148 subunit interaction, 394-395 synthesis and cAMP level, 144 Alkylated DNA and E. coli exonuclease
II, 221
Alkylating agents, 111-112, see also indi
vidual compounds Arthus reactivity and, 123 bifunctional, 224-225
granulocyte suppression and, 123 monofunctional, 225
nucleic acid synthesis and, 176 polymorphonuclear leukocyte and, 123 Allosteric effector, 397
guanidine, 242
Allosteric effects in amino acid trans
port, 79
Allosteric inhibition, 72 amino acid transport and, 72 Allosteric protein, 397
Allosteric regulation and amino acid bio
synthesis, 148
Amanita phalloides toxin, 181 a-Amanitin, 181-182
RNA polymerase and, 154 Amantadine
influenza virus and, 238
mechanism of antiviral action, 239-240 virus replication and, 238
Amber mutations and λ repressor, 141 AMD, see Actinomycin D
Amicetin, 337
Amines, antiviral activity of, 240 Amino acid(s)
absorption, concentration and, 74 iV-methylated, transport of, 74-75 molecular size and, 74
fatty acids and, 91-92 nerve growth factor and, 93 temperature dependence of, 98-99 activation, inhibitors of, 333 biosynthesis
control, 148-151 derepression and, 149 carrier, effect of cations, 86 cis-cis interactions of, 77-79
allosteric effects, 79 competition, 78
competitive stimulation, 79 multiroute transfer, 78-79 cis-trans interactions of, 80-82
competitive stimulation, 81-82 countertransport, 80-81 compartmentalization of, 86 Amino acid starvation, 227 Amino acid transport, 9
acetylcholine and, 87 actinomycin D and, 88, 178
amino acid accumulation, control of, 92-94
amino acid interactions in, 77-82, 77-79, 80-82, 79-80
amino acid structure and, 73-75 D and L amino acids, 74 amino group, importance of, 74 ammonium ion, effect of, 87 calcium and, 87-88
carboxyl group, importance of, 74 cardioactive glycosides and, 9
cell volume and, 94
competitive stimulation, 81-82 control of, 70-73
allosteric inhibition and, 72 competition for transfer site and,
72
energy, availability of, 72-73 membrane permeability, changes in,
72
protein metabolism and, 73 structure and, 71
countertransport, 80-81 cyclic behavior of, 100 diet and, 100
energy considerations, 97-100 environmental changes and, 89 eserine and, 87
D-galactose and, 89-92 genetics and, 73, 100-101 glucagon and, 94 L-glutamate and, 87
hormones, influence of, 94-97 lipid, effect of, 91-92 metabolic inhibitors and, 97 metabolic pathways and, 99-100 Ν -methylated amino acids, 74-75 model systems, 80
Na-ATPases and, 83
negative feedback inhibition and, 92 nitrogen starvation and, 92 nonmetabolizable sugars and, 91 osmotic shock and, 89
other solute transport systems and, 89-92
ouabain and, 84, 87 oxygen availability and, 94 pH and, 88
positive feedback and, 93 potassium ion and, 86-87 puromycin and, 88
side chain substituents, effect of, 75 sodium ion and, 82-86
sugar transport, interaction with, 89-92 sulfur starvation and, 92
systems for, 75-77 temperature and, 98-99 trans inhibition of, 92 triiodothyronine and, 96
«-Amino acids, 75 β-Amino acids, 75
Amino groups, succinylation of, 280 Aminoacyl transfer RNA, 259 Aminoacyladenosine, 259 2-Aminobicyclo-[2,2.1]-heptane-2-
carboxylic acid, 80
Aminobutyric acid, valyl-iRNA synthe
tase and, 150 1-Aminocyclopentane, 82
SUBJECT INDEX 473
Aminoglycosides 30 S subunit and, 333, 334
α-Aminoisobutyric acid, 75, 317 2-Amino-3-methylthiobutyric acid, 150 4-Aminoquinoline compounds, 195 Ammonia, glucose and production of,
142
Ammonium ion
amino acid transport and, 87 antiviral activity of, 240
Amoeba pro tens, nuclear transplantation in, 164
Amphiprotic compounds, amino acid transport and, 88
Amphotericin B, 65 Amytal, 34
Anaerobic glycolysis, actinomycin D and, 178
Anaerobic tissues, LDH isozymes in, 401 Anaphylaxis, antibody and, 118
Androgens, gene derepression and, 160 Anesthetics, 347, see also specific
compounds
NaK-ATPase, effect on, 34 Annealing of DNA, 212-213
Anoxia, amino acid transport and, 97-98 Anterior pituitary gland, 367
Anthracyclines, 196, 325-326 binding with DNA, 176 Anthramycin, 344
binding to DNA, 326
Anthranilate synthetase, derepression and,151
Antibacterial antibiotics, 65-66 Antibiotics, 111, see also specific com
pounds
antibacterial, 65-66 antifungal, 65-66 binding to proteins, 344 channel-forming, 65-66
chemical structure and biological ac
tivity of, 342-346 interaction with DNA, 344 limits of specificity, 346 origin and function, 346-348 virus increase and, 290 Antibody
anaphylaxis and, 118 Arthus reactivity and, 123
formation, immunosuppressant pre- treatment and, 124
immune lysis and, 118 responses, 109, 118, 119 synthesis, 118
Antiepileptic agent, diphenylhydantoin, 35
Antifungal polyene antibiotics, 65-66 Antigen (s)
administration timing of, 115-116 Arthus reactivity and, 123 immunological tolerance and, 122 immunosuppressive regimens and, 115 Antigen-antibody reaction, 118 Antihemophyllic globulin, immunosup
pressants and, 129 Antiinducers, 139
Antilipolytic activity, insulin and, 367 Antilymphocyte serum, 111, 113
action of, 125
renal homograft and, 127 toxicity of, 129
Antimalarial compounds, 195 Antimetabolites, 111, 113, see also indi
vidual compounds psoriasis and, 129 toxicity of, 129 Antimycin A, 320 Antiport, 316
Antipurines, cancer chemotherapy and, 113
Antipyrimidines
cancer chemotherapy, 113 effects of, 113
Antirepressor, cro gene and, 142 Antitumor antibiotic
mitomycin C, 191 streptonigrin, 196
Antitumor substance, sarkomycin, 193 Antiviral chemicals, 278
Antiviral factor, 282-283 mode of action of, 289 Aphids, 287
Aporepressor, 417
Ara-A, see Arabinosyladenine Ara-ATP, DNA polymerase and, 192 Ara-C, 266-267
mechanisms of action, 266-267
treatment of herpes keratitis, 266 Ara-CPT, DNA polymerase and, 192 1 /3-D-ArabinofuranosyIcytosine, 266-267 Arabinose operon
derepression of, 149
positive control system and, 147 Arabinosyladenine, phosphorylation of,
192
D-Arabinosyl nucleosides, 176 Arachidonic acid, 98
Aranotin, 258 Arboviruses
actinomycin D and, 251 replication of, 242 Arginine
arginyl-iRNA and, 151 histones and, 155 transport, 82
osmotic shock and, 89 Arginine phosphate, 2 Arginine-rich histones, 157 Arginyl-tRNA, arginine and, 151 Aromatic rings, π-π interactions, 55 Arsenicals, 20
Arsenite, effect on NaK-ATPase, 22-23 Arthus skin reaction, cause of, 123 Artificial lipid bilayers, valinomycin and,
315
Artificial membranes, 75
nonelectrolyte permeability of, 75 ASC, 76
Ascites cells, 76
Ascites hepatoma cells, 188 Ascites tumor(s)
chromosome RNA and, 158 nucleoprotein, histones of, 157 Aspartate, 385
Aspartate transcarbamylase, 397 Aspartokinase isozymes, differential
feedback inhibition and, 385 Aspartyl phosphate
lysine biosynthesis and, 385 methionine synthesis and, 385 threonine biosynthesis and, 385 Aspergillus flavus, 186
Aster yellows mycolpasma, 299 7 -[3 2P]ATP, 4
ATP hydrolysis calcium dependent, 7
ITP, competitive inhibition by, 6 ATP synthesis, inhibition by oxidative
phosphorylation uncouplers, 63 ATPase, antibiotics and, 322-323 Atropine, NaK-ATPase, effect on, 34 Aurantin, 188
Autoimmune allergic encephalomyelitis, cyclophosphamide and, 128 Autoimmune disease, 110, 119, 128-129 Autosomes, 101
Avenaciolide, 342 AVF, see Antiviral factor Avian myeloblastosis virus, 251
reverse transcriptase of, 163 Avian RNA tumor viruses, 251
Avian sarcoma viruses, amantadine and, 239
8-Azaguanine, 294
alfalfa mosaic virus and, 297 Azalomycin, 313
Azaserine, 111 activity of, 113 Azathioprine, 111
differential effectiveness of, 118 renal homograft and, 126 therapeutic use of, 113 Azide, 62
Β
Bacillus megaterium, chloroquine and viability of, 195
Bacillus subtilis cordycepin and, 184 endonucleases of, 221
Bacillus subtilis phage SP01, RNA spe
cies production in, 153 Bacitracin
cytoplasmic membrane and, 309 mycoplasmas and, 299
Bacterial chemotaxis, 89 Bacterial episomes, 217 Bacterial membranes
ATPase activity in, 12 cardioactive glycosides and, 12 phosphorylytic systems, activation of,
12
SUBJECT I N D E X 475
Bacterial plasmid (Col. E i ) , inhibition of replication of, 163
Bacterial RNA polymerase, rifamycin and, 179
Bacteriocins, proteinaceous, 306 Bacteriophage lambda, 231
Bacteriophage 0X174, initiation proteins and replication of, 162
Bacteriophage S13, 162
Bacteriophage T4, σ factor and, 166 BAL, 22
Barbiturates, 321 Base ratio analysis, 255 BcS, 292
Bean pod mottle virus, actinomycin D and, 291
Bean yellow mosaic virus, 280 Beauvericin, 315
Berninamycin, 338 Beryllium ions
Mg-ATPase, effect on, 32 NaK-ATPase, effect on, 32-33 Beryllium-NaK-ATPase complex, 32 Biguanide compounds, influenza virus
and, 238
Binary complex in amino acid transport, 83
Biotin transport, uranyl ions and, 61 Bisacodyl, 37
Blasticidin S, 292
Blastocladiella emersonii, rifampicin and, 181
Bleaching of Euglena, myxin and, 329 Blue diaper syndrome, 100-101 Boromycin, 342
Boron-containing antibiotic, 342 Borrelidin, 345
amino acid activation and, 333 Bovine LDH, 388
Bovine lymphosarcoma, 192 Bovine serum albumin
antigen strength of, 116
immunosuppression by 6-mercapto
purine, 118
Branched-chain amino acid biosynthesis, repression control of, 150
Brassica pekinensis, 296 Bromegrass mosaic virus, 293 Bromide, NaK-ATPase and, 31
5-Bromo-2'-deoxyuridine, 113, 262-265 phosphorylation of, 264
5-Bromouracil, tobacco mosaic virus and, 295
Bryamycin, 338
BUDR, see 5-Bromo-2'-deoxyuridine Bufadienolides, 9
Busatin, 246
iV-Butylmaleimide, 21
C
Ca-ATP complex, 7 Ca-ATPase, 7
(Ca plus Mg)-activated ATPase, effect of 2,4-dinitrophenol, 29
Cabbage virus B, 284 Cadmium ion, toxicity of, 35
Calciferol metabolite, gene derepression and, 160
Calcitonin, 363 Calcium
amino acid transport and, 87-88 extrusion from erythrocytes, 12 transport, 7
Calcium-dependent ATPase, 7 Calf thymus polymerases A and B, strep
tolydigin and, 185 cAMP, 364, 366, 367
alkaline phosphatase synthesis and, 144
binding protein, 146-147 catabolite repression and, 144 cellular level, control of, 144 DNA-/ac repressor binding and, 139 /3-galactosidase synthesis and, 144-145 glucose effect and, 144
lysogeny regulation and, 145 receptor protein, 146 site of action of, 145
transient repression and, 144-145 tryptophanase production and, 144 Cancer
chemotherapy, 110-111, 113, 197 RNA virus induced, 197 Candida, 321
iV-Carbamyl-O-acetyl oxytocin, 373 Carbohydrate metabolism, amino acid
transport and, 99-100
Carbohydrate transport, insulin and, 367 Carbomycin, mycoplasmas and, 299 Carbonate ion, LDH isozymes and, 400 Carbonic anhydrase, 60
Carbonylcyanide-m-chlorophenylhy- drazone, 62
5-(Carbophenoxyamino) salicylic acid, LDH isozymes and, 418-419 Carboxyl groups, binding with uranyl
ion, 61
Carboxylic polyethers, 316-318 Carboxypeptidase, 397
Carcinogenesis, RNA viruses and, 197 Carcinogenic agents, anatoxins, 186-187 Carcinogens, nucleic acid synthesis and,
176
Cardenolide derivatives, 9, 10 Cardiac glycosides
effect on cardiac microsomal ATPase, 12
inhibition of K+ dependent phospha- tase activity, 13
inhibitory site, 9
Na-dependent amino acid transport and, 84
rate of action, dose dependence, 15 Cardioactive steroids, 8-18
alteration of membrane configuration by, 13
effect on sodium transport, 8 3-haloacetate derivatives, 24-25 hellebrigenin, 24
3-haloacetate, 24-25 inhibitory site, 9
membrane and microsomal Mg-ATPase and, 11
membrane and microsomal NaK- ATPase and, 11
NaK-ATPase, interaction site, 9 sodium transport and, 8
strophanthidin, 3-haloacetate, 24-25 structure of, 9
Carnation, 281
Carzinophilin, DNA synthesis and, 194 Casein production
hydrocortisone and, 157 insulin and, 157 prolactin and, 157 Cassaine, 19
Catabolite gene activator protein, func- tion of, 146, 147, 166
Catabolite repression, 139, 142-147 cAMP control of, 144-146 transient repression of, 143-144 Catalase, subunit interaction, 394 Catalase isozymes, sulfhydryl reagents
and, 398
Catecholamine transport, 9 cardioactive glycosides and, 9 Caterpillars, 287
Cation transport, 2-5 NaK-ATPase and, 2-5
NaK-ATPase in erythrocyte ghosts, 4
Cationic amino acid transfer, inhibition of, 91 transport, 80
Cauliflower mosiac virus, 291 CCCP, 62
Cell differentiation, gene derepression and, 159
Cell division
control by thymidine, 164 hydroxyurea and, 189 phleomycin and, 193 streptonigrin and, 196
Cell growth 5-halogen-2'-deoxyuridines and, 263-264
Cell leakage, 57
polyene antibiotics and, 57 Cell membrane
asymmetric distribution of sodium, 84 lipid phase, 56
interaction of lipid bilayer with di- phenolic compounds, 56-57 interaction with phlorizin and
phloretin, 55 structure
sugar transport and, 64-66 Cell proliferation, hydroxyurea and, 189 Cell structure, 73
Cell surface combination with uranyl ions, 61
Cell wall synthesis, inhibitors of, 307-311 Cellobiose, 59
Cellular immune response, 124-126 alkylating agents and, 125 Arthus reactivity and, 123
SUBJECT I N D E X 477
destruction by corticosteroids, 125 immunological tolerance and, 125 lymphocytes and, 125
X-irradiation and, 125 Cephalosporins, 309 Cereal viruses, 295
Cerebrospinal fluid formation, 20 Cestodes, proline absorption by, 90 CRF, see Chlorpromazine free radical cGMP inhibition of cAMP-protein bind
ing, 146 Chalones, 361
depression of mitotic activity, 368 Channel formation, polyene antibiotics
and, 57
Channel-forming antibiotics, 65-66 Charge neutralization, amino acid solu
bility and, 89
Chelation of Mg by ATP, 6 Chelating agents
membrane permeability and, 87 phage particles and, 211
Chemical mutagens, nucleic acid synthe
sis and, 176
Chemiosmotic hypothesis, 62, 313 Chemotaxis, 89
Chenopodium amranticolor, 281 Chetomin, 258
Chikungunya virus, actinomycin D and, 254
Chinese hamster cells, 190 SV40 virus and, 267
Chi tin synthesis, inhibitors of, 311 Chi tin synthetase, 345
polyoxin D and, 311 Chlorambucil, 112
immunosuppression and, 112 Chloramphenicol
β-galactosidase synthesis and, 145 gene replication and, 162 mycoplasmas and, 299 protein synthesis and, 120 50 S subunit and, 333, 336
Chloramphenicol-resistant proteins, gene replication and, 162-163
Chlordane, 33
Chlorinated hydrocarbons, see also spe
cific compounds
NaK-ATPase, effects on, 33
Chlorite, 209 Chlormerodrin, 21
p-Chloromercuribenzoate, 20, 63 D N A polymerase and, 193 sugar transport and, 57-58 p-Chloromercuriphenylsulfonate, 21 Chloroplasts, valinomycin and, 315 Chloroquine, 195-196
D N A and absorption spectrum of, 195 intercalation with DNA, 176 Chlorpromazine, 20
NaK-ATPase, effect on, 25 ultraviolet radiation and, 25-26 Chlorpromazine free radial
K-dependent phosphatase, effect on, 25-26
Chlorpromazine free radical Mg-ATPase, effect on, 26 NaK-ATPase, effect on, 25-26 Chlortetracycline, mycoplasmas and,
298-299
Cholecystokinin, 363 Cholesterol, 56 Choline, 243
Chromatin RNA synthesis, actinomycin D and, 177
Chromomycin, 188
DNA complex with, 176, 326 Chromomycin A3
interaction with guanine, 188 RNA synthesis and, 188
Chromosomal aberrations, induction of, 188-189
Chromosome puffing, RNA synthesis and, 161
Chromosome RNA (cRNA), source of, 158
specific repression and, 158 Chromosomes, chemical and physical
measurements of, 212
Chronic hepatitis, immunosuppressants and, 129
Chymotrypsin, 38, 397
phosphotransferase, destruction of, 65 viral inhibitors and, 281
Chymotrypsinogen, 397 cl gene
immunity and, 140 lysogeny and, 140
transcription promoters and, 142 Cinerubin
DNA complex with, 176 nucleic acid synthesis and, 196 Circular DNA, and ethidium bromide,
195
Citrate synthetase, isozymes of, 398 Clathrates, 343
Clostridia, streptolydigin and, 185 Clover phyllody mycoplasma, oxytetra
cycline and, 299-300 Coat-stripping of virus, 289 Coelomic amino acid levels, 94 Coenzyme Q, 320-321
Colchicine, DNA synthesis and, 194 Colicin, nucleic acid synthesis and, 196 Colicins (Ei and K), 63
β-galactoside uptake and, 63 Colistins, lipid membranes and, 311 Compartmentalization
of amino acids, 86 of sodium ion, 86
Configurational isozymes, 396 Conformational changes
in NaK-ATPase, ligand induced, 38 translocation process and, 59
Congenital cytomegalovirus, IUDR and, 263
Conjugal fertility factor F, 214 Constitutive enzymes
glucose effect and, 142 production of, 137
Constitutive mutants, transient repres
sion and, 144
Copper chloride, effect on sugar and gly
cerol transport, 60-61
Copper-containing peptide antibiotics, 193
Copper ion, toxicity of, 35
Copper transport, amino acids and, 89 Cordycepin, 184-185
Cordycepin triphosphate, 184 Cordyceps militaris, 184 Core polymerase, 180 Corepressor, 417
histidine biosynthesis and, 149 Corpora lutea, 366
Corticosteroids, autoimmune disease and, 128
Cortisol, binding to histones, 160 Cortisone, chromosome puffing and, 161 Coumarin-3-carboxylic acid, LDH
isozymes and, 418 Coumingine, 19 Countertransport, 90 Cowpea chlorotic mottle, 286
Cowpea chlorotic mottle virus, thiouracil and, 296
Cowpea plants, 280
Cowpox virus, rifampicin and, 247, 249 Coxsackie A and Β viruses, 242 Coxsackie virus B6, rifampicin and, 249 Crab nerve, microsomal fraction of, 2 Creatine phosphokinase, subunit interac
tion, 394-395
cro gene, antirepressor and, 142
CR-protein, see cAMP, receptor protein cRNA-histone effect, RNA synthesis
and, 158
Crossovers, unequal, 229
CTP, substrate for NaK-ATPase, 6 Cucumber mosaic virus, 280, 286 Cyanide, 2, 16
Cyclic AMP, see cAMP Cyclic GMP, see cGMP Cyclodepsipeptides, 313, 319 Cycloheximide
AIB uptake and, 95 mechanism of action, 259 protein synthesis and, 258
Cyclohexylenediaminetetraacetate, 16 Cycloleucine, 90, 100
thyroidectomy and, 96 transport, galactose and, 91 Cyclophosphamide, 111, 122
autoimmune disease and, 128 differential effectiveness of, 118 immunosuppression and, 112 nonspecificity of, 120
timing of administration, 115-116 Cycloserine, 343
mycoplasmas and, 299 Cymarin, 11
cysC region, 217
Cysteine, sarkomycin and, 193 Cystinuria, 101
Cytochrome b, 320-321 Cytochrome bi, 50, 62
SUBJECT I N D E X 479
Cytochrome c, 320-321 Cytochrome oxidase, 320
Cytopathic changes, virus induced, 244 Cytoplasmic DNA, hydroxyurea and,
190
Cytoplasmic inheritance, ethidium bro
mide and, 194
Cytoplasmic membrane, bacitracin and, 309
Cytoplasmic repressore, regulatory genes and, 137
Cytoplasmic RNA synthesis, actinomy
cin D and, 177
Cytosine, deamination by nitrous acid, 224
Cytosine arabinoside, 266-267 DNA synthesis and, 255
Cytotoxic antineoplastic drugs, 110, see also Immunosuppressants
D
Danthron, 37 3'-dATP, 184
Datura stramonium, 283 Daunomycin, 196, 325
absorbance of, DNA and, 196 DNA complex with, 176 p,p'-DDE, 33
DDT, 33 ο,ρ'-DDT, 33
9-Deamidolysine vasopressin, 372 Dehydrogenases, 62
Dehydrogliotoxin, 258
Delayed hypersensitivity, 118, see also Cellular immune responses genetic factors and, 119 in guinea pigs and man, 119
suppression by 6-mercaptopurine, 118 suppression by methotrexate, 122 Deleted genes, rescue and detection of,
213-216
Deletion-inducing agents, 224-225 Deletion mapping, 206
Deletion mutants, 206
frequency of occurrence, 207, 227 frequency, topography, and topology
of, 216-221
mechanisms for, 226-230 origins of, 207
UV-induced, 219 Deletions
acridine-induced, 226 continuity of, 216 interstitial, 221 terminal, 221
iV-Demethylrifampicin, 197 3'-Deoxyadenosine, 184
3'-Deoxyadenosine 5'-triphosphate, 184 3-Deoxy-D-ara6ino-heptulosonate-7-
phosphate synthetase, 151 Deoxycholate, 12
NaK-ATPase, effect on, 36-37 Deoxycytidylate deaminase allosteric
feedback inhibition of, 264 2-Deoxyglucose, 58
transient repression and, 144 Deoxyguanosine
actinomycin and, 177 Deoxyguanylic acid, 325 3-Deoxypentose, 184 4-Deoxyphloretin, 54 Deoxypyridoxine, 93
Deoxyribonuclease, cellular, activation by mitomycin C, 191
Deoxyribonucleotide formation, hydroxy
urea and, 189 Dephophorylation
of lipid carrier, 309
of NaK-ATPase by hydroxylamine, 17 Depolymerization of DNA, mitomycin
C and, 191 Depurination, 225
Derepression and histidine synthesis, 149 Derepressors, 159-161, 166, 167
Dermatomyositis, 129 Desertomycin, 313 Deshistidine glucagon, 377 Desmosomes, 87
Detergents, see also specific compounds membrane lipids, effect on, 64 NaK-ATPase, effect on, 36-37 DFP, 31-32
DHU, 158
7V,0-Diacetyl oxytocin, 372 Dianemycin, 316
Dianthus caryophyllus, 281 Diauxie, 142
Dibasic amino acid transport, 82 iV,0-Dicarbamyl oxytocin, 372
p,p'-Dichlorodiphenyltrichloroethane, 33 N,iV'-Dicyclohexylcarbodiimide, 12 4,6,-Dideoxyphloretin, 54
Dieldrin, 33
α,α-Diethylglycine, 76
Diethyl-p-nitrophenyl phosphate effect on NaK-ATPase, 32
Diethylstilbestrol, 36
K+-dependent phosphatase, effect on, 36
NaK-ATPase, effect on, 36 sugar transport, effect on, 55
Differential feedback inhibition, asparto- kinase isozymes and, 385
Differentiation
control by hormones, 161 operon systems and, 155
of ganglia, nerve growth factor and, 93
Difocol, 33 Digitalis, 9
Digitonin, effect on membrane lipids, 64
Digitoxigenin, 10 Digitoxin, 15 Digoxin, 15
Dihydrofolate reductase, methotrexate and, 120
20a-Dihydroprogesterone, 365 Dihydrostreptomycin, 345 Dihydrouracil, 158
Dihydroxyacetone phosphate, 385 Dihydroxyurea, 190
Diisopropylfluorophosphate, effect on NaK-ATPase, 31-32
2,3-Dimercapto-l-propanol, 22 6-Dimethylamino-9-[3-deoxy-3-(p-
methyl-L-phenylalanylamino)-j3-D- ribofuranosyl] purine, 259 Dimethylethanolamine, 243
3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2- hydroxyethyl] glutarimide, 258 Dimethylpropanolamine, 243 m-Dinitrobenzene, 59
F D N B inhibition and, 59-60
2,4-Dinitrophenol
(Ca plus Mg)-activated ATPase and, 29
mitochondrial ATPase, effect on, 29 oxidative phosphorylation and, 29 Dio 9, 322-323
Diphenolic compounds mechanism of inhibition, 56-57 sugar transport, effect on, 56-57 Diphenylhydantoin, 35
Diploid organisms, deletion mutants in, 206
Distamycin, 327
RNA polymerase and, 153 Diterpene acids, aminoethyl esters,
iV-alkyl derivatives of, 19
Dithiothreitol, D N A polymerase and, 193 Diuresis, 8
ethacrynic acid and, 23 Diuretics, 20-21
Divalent cation transport, uranyl ions and, 61
DNA
binding proteins, D N A replication control and, 164-165
biosynthesis, 175-197 bushes, 213
cross-linking agents, 224-225 cross-links, nitrous acid and, 224 fragmentation
hydroxylamine and, 188 hydroxyurea and, 188 heterologous strands, 212
hydrolysis, backbone cleavage, 225 ligase, 223, 226
looped-out regions of, 213 loops, 213
melting of, 330 melting temperature
chloroquine and, 195 nogalamycin and, 187 polymerase
acriflavine and, 195 dithiothreitol and, 193 ethidium bromide and, 195 phleomycin and, 193 streptonigrin and, 196 sulfhydryl reagents and, 193 synthesis, inhibition by histone, 164
SUBJECT INDEX 481
polymerase I, 223 repair enzymes, 207 replicases, 226 replication
immunosuppressants and, 119 inhibitors of, 323-331
synthesis
alkylating agents and, 120 cyclophosphamide and, 120 5-halogen-2'-deoxyuridines and,
263-264
hormones, effect of, 94 6-mercaptopurine and, 120 methotrexate and, 120 phleomycin and, 193 reverse transciptase and, 163 steric hindrance, inhibition by, 191 viruses, actinomycin D and, 255 DNA-dependent RNA synthesis, actino
mycin inhibition of, 176-178 DNA-histone complex, 159
D N A : R N A ratio, hydroxyurea and, 189 DNase, 191
Docosahexanoic acid, 98 Dodecyl sulfate, 29, 37 Drosophila melanogaster
alcohol dehydrogenase of, 389 enzyme polymorphisms in, 395 Dyes, virus increase and, 290
Ε
Ecdysone, chromosome puffing and, 161 Echo virus, 242
rifampicin and, 249
Eczema vaccinatum, methisason and, 244 Edeine, DNA synthesis and, 194 EDTA, effects on phage, 211 Effectors, and repressors, 137
Ehrlich ascites carcinoma cells, 177, 193 streptovaricin and, 186
Electrical conductance, macrotetrolides and, 316
Electron microscopy, 87
Electron micrographs, length of DNA and, 212
Electrophoresis, enzyme heterogeneity and, 389, 391
Electrophorus, electric organ, 21 NaK-ATPase of, 17
Enantiomorphic amino acids, transport of, 74
Encephalomyocarditis virus, rifampicin and, 249
Endo mechanism, 417 Endonuclease, 221-224
of Bacillus subtilis, 221 of Micrococcus luteus, 221 UV-specific, 224
Endoplasmic reticulum biosynthesis effect of hormones, 94
Enduracidin, peptidoglycan biosynthesis and, 310
Energy
availability of, 72
amino acid transport and, 72 metabolism, inhibition of, 61-63 transfer, inhibitors of, 319-323 Enniatin(s), 314-316, 343
Environmental temperature, 98-99 Enzyme heterogeneity, 388, see also
Isozymes
multiple forms and, 395 Enzyme-mediated ligation, 226 Enzyme multiple forms, genetic cause
of, 395
Enzyme polymeric states, 396
Enzyme polymorphisms, isozymes and, 395
Emimycin, 341 Emulsin, 285
Epigenetic factors, LDH isozymes and, 392-393
Epinephrine
AIB uptake and, 94 lipolytic action of, 366 Episomes, 162, 214
Erythritol transfer, calcium and, 88 Erythrocytes, 8
active center of K+-dependent phos
phatase, 14
calcium extrusion from, 12 facilitated diffusion in, 58
ghosts, resealed, sodium transport, re
lationship of NaK-ATPase to, 2-5 Erythromycin, 337, 345
50 S subunit and, 333
NaK-ATPase, effect on, 28 Erythrophleine, 19
Erythrophleum alkaloids, effect on NaK-ATPase, 19-20
Escherichia coli
active transport of sugars in, 62 aspartokinase isozymes and, 385 chlorate-resistant mutants, 209 deletion mutants of, 217-218 /3-galactoside transport in, 49 lac permease of, 57
Tl-resistant mutants, 208 Eserine, 87
Esterases, 383
Estradiol, AIB transport and, 95 Estrogenic activity, 55
Estrogens
amino acid transport and, 95 gene derepression and, 160 Ethacrynic acid, 20
ATP production, effect on, 24 cysteine sulfhydryl group, reaction
with, 23
NaK-ATPase, effect on, 23-24 sodium extrusion, effect on, 24 stability of phosphorylated NaK-
ATPase and, 24 Ethanolamine, 243
Ethidium bromide, nucleic acid synthesis and, 194
Ethionine, 76, 243
Ethyl alcohol, amino acid transport and, 97
N-Ethylhydroxyurea, 190 iV-Ethyl-ITSC
N-Ethylmaleimide, 63-64 ATPases, effect on, 11 DNA polymerase and, 193 phosphoenolpyruvate phosphotrans
ferase and, 58 sugar transport and, 57-58 Euchromatin, histones of, 157 Eukaryote chromosomes, 163-165 Eukaryote transcription, 154-161 Euthyroid, 96
Exchanging carrier (L system), 81 Exo mechanism, 417
Exonuclease II, alkylated DNA, speci
ficity for, 221
Exonucleases, 221-224
Extracellular amino acid concentration, constancy of, 100
Extracellular-intracellular sodium ex
change, 5
F
Facilitated transfer, basic assumptions of, 81
p Factor, 141
function of, 153, 167 σ Factor, 152, 166
RNA polymerase and, 146, 166 φ Factor, (^β replicase and, 153 Factor TF11, 341
Fatty acids
amino acid accumulation and, 91-92 in membranes, temperature depen
dence of, 98-99
FDNB, see Fluorodinitrobenzene Fertility factor F, 214, 229 Fibroblasts, 86
Filamentous growth, myxin and, 328 Flavensomycin, 322
Flavin mononucleotide, 71 tryptophan, complex with, 71 Fluoride, 30-31, 99
glycolysis, inhibition of, 30 K-dependent phosphatase, effect on,
31
Mg-ATPase and, 31
NaK-ATPase, effect on, 30-31 Fluoroacetate, 99
5-Fluorodeoxyuridine, DNA synthesis and, 226
Fluorodinitrobenzene, 58-60 ethanol, urea, detergents, and, 59 glucose and, 58
inhibition kinetics, 58-59 p-Fluorophenylalanine, tryptophan
transport and, 79 5-Fluorouracil, 113, 263, 294
tobacco mosaic virus and, 296 FMDV, see Foot-and-mouth disease
virus
Focus formation, rifampicin inhibition of, 249
SUBJECT I N D E X 483
Folic acid antagonist, 111 cancer chemotherapy, 113
Follicle-stimulating hormone, amino acid transport and, 95-96
Foot-and-mouth disease virus, 242 actinomycin D and, 253
Formaldehyde, lac permease and, 58 Formamide, renaturation of DNA and,
213
Formamidoxime, 190 Formycin, 340
Formycin triphosphate, 340 Formylated methionyl-iRNA, 336 Fowl plague virus
actinomycin D and, 252-253 amantadine and, 240 Foxglove, 9
Frame-shift deletions, 228 Frame-shift mutations, 228-229
acridine induced, 229
induction and reversion of, 228 Fructose 1,6-diphosphate, 385
heat inactivation of LDH and, 413 FSH, 95-96
F3TDR, 264-266
D-Fucose, ara C gene protein and, 148 FUDR, 226
phosphorylation of, 263
Functional genome unit, gluconeogenesis and, 155
Fungal cell walls, 311 Furanomycin, 333 Fusidic acid, 338, 346
G G enzyme, 149 G factor, 338 GABA, 76 gal operon, 210 Galactokinase, 209-210
Galactose, cycloleucine transport and, 91
D-Galactose amino acid absorption and, 89
Galactose epimerase, 209 Galactose-negative mutants, 210 Galactose 6-phosphate, 51
Galactose-transducing phage Xdg, 219 Galactose transport, 51
β-Galactosidase
glucose effect and, 143 inducibility, 136, 138, 165 induction, kinetics of, 143
synthesis, chloramphenicol and, 145 /3-Galactosides, 49
Galactoside permease in E. coli, 210 Ganglia, growth and differentiation of,
93
Gastrointestinal hormones, 364 Gene
derepression, hormones and, 160-161 pickup, 214
regulation model, 165 replication
chloramphenicol and, 162 initiation proteins and, 162 Gene N, 140
Genetic deletions, 205-231 Genetic homology, 211 Genetic maps, 216
Genetic recombination, control of, 161 Gliotoxin
toxicity for animals, 257 virus replication and, 257 Glomerulonephritis, 129 Glucagon
adenyl cyclase and, 377 AIB transport and, 95 amino acid uptake and, 94 cyclic AMP and, 367 D-Glucan, - > 3)-linked, 284 Glucocorticoid hormone, 368
Glucocorticoids, gene derepression and, 160
Gluconeogenesis, 385 enzymes and genome, 155 insulin and, 155
Glucose effect, 142-143 adaptive enzymes and, 142 constitutive enzymes and, 142 cyclic AMP and, 144
definition of, 142
transport mechanisms for, 51 Glucose-6-phosphate dehydrogenase, in
liver and kidney, 389
Glucuronide, phenolphthalein mono-, 37
Glutamate
polymeric glutamic dehydrogenase and, 396
transport, pH and, 88
L-Glutamate, amino acid transport and, 87
Glutamic dehydrogenase (GDH), poly
meric states of, 396
Glutamine synthetase, isozymes of, 398 7 -Glutamyl phosphate, NaK-ATPase re
action intermediate, 29 Glutathione, sarkomycin and, 193 Glyceraldehyde 3-phosphate, 385 Glycerol phosphate, 310 Glycerol transport, 60-61 Glycine transport
D-galactose and, 89 potassium and, 87 sodium ion and, 83-84 uranyl ions and, 61
Glycogen phosphorylase, isozymes of, 398
Glycogen synthetase, isozymes of, 398 Glycolysis, 385
inhibition by fluoride, 30 in tumor cells, 97
Glycoprotein, viral inhibitors, 280 Glycoside antibiotics, daunomycin, 196 Glycylamidosalicyclic acid, LDH iso
zymes and, 418 Gold chloride, 60-61 Gomphrina globosa, 282-283 Gougerotin, 337
Gradient-coupled sugar transport, sec
ondary active transport, 50 Gram-positive bacteria, streptovaricin
and, 186 Gramicidin A, 65-66 Gramicidins, 318-319
alkali metal diffusion and, 314 induction of ion transport, 319 Granaticin, 327, 344
Granulocyte
azathioprine and, 126
suppression, alkylating agents and, 123 Growth factors, 93
sulfate incorporation and, 93-94 Growth hormone, 362
GTP, substrate for NaK-ATPase, 6
Guanidine
as allosteric effector, 242 antiviral activity of, 241-243 antagonists, 243
salts, animal RNA viruses and, 293 Guanidine carbonate, virus infection
and, 294
Guanidine hydrochloride, LDH isozymes and, 391, 394
Guanine
biosynthesis, mycophenolic acid and, 340
deamination by nitrous acid, 224 interaction with chromomycin A3, 188 Guanosine tetraphosphate, lac mRNA
production and, 147
Guanosine triphosphate, 332, 338, 346 Guinea pig cerebral cortex, 17 Guinea pig kidney medulla, NaK-
ATPase activity in, 5
Η Hallucinogens, 347
Haploid organisms, deletion mutants in, 206
Haploids, lethality of mutations in, 216 HBB, 241-243
HCG, 363 her loci, 228 HCS, 363 Heart
immunological resistance, 119 microsomes, Mg2 +-ATPase and NaK-
ATPase in, 12
Heat resistance, phage T5, 211 Heavy-metal ions, sugar and glycerol
transport, 60-61 Hedamycin
DNA synthesis and, 327 nucleic acid synthesis and, 196 HeLa cells, D N A replication in, 163 Hellebrigenin, 3-haloacetate derivative,
24-25
Helvolic acid, 338
Hemicholinium-3, NaK-ATPase, effect on, 34
SUBJECT I N D E X 485
Hemicholinium HC-3, 243
Hemoglobin, a- and β-chain genes of, 155
Hemolytic anemia corticosteroids and, 128 immunosuppressants and, 128 thiopurines and, 128
Henbane mosaic virus, 294 HEP-2 cells, 253
Hepatotoxins, 186
Herpes simplex encephalitis, IUDR and, 263
Herpes simplex virus, 262 Herpes virus, rifampicin and, 249 Heterochromaticity, chromosome tran
scription and, 156
Heterochromatin, histones of, 157 Heteroduplex molecules, 213
Heterocyclic compounds, rhinovirus and, 246
Hexestrol, effect on NaK-ATPase, 36 Hexose metabolism, effect of potassium,
86
his operon, repression by 1, 2, 4-triazol, 149
Histidine, 74
biosynthesis, operon system of, 148 copper, reaction with, 61
operons, 210
transport, feedback inhibition of, 93 Histidyl-tRNA, his operon repression
and,149, 166
Histidyl-tRNA synthetase, repression of his operon and, 148
Histocompatibility, kidney transplanta
tion and, 128 Histone (s)
action of, 156 classification of, 156
DNA polymerase synthesis and, 164 of euchromatin, 157
gene inertness and, 156 of heterochromatin, 157 repression
cRNA and, 158
nonhistone proteins and, 158 repressors, 155-158
synthesis
hydrocortisone and, 157
hydroxyurea and, 189 insulin and, 157 prolactin and, 157
H-2 locus, tissue transplantation and, 117
HL-A locus, tissue transplantation and, 117
Hong Kong virus, amantadine and, 240 Hormone (s)
amino acid transport and, 94-97 analogs
naturally occurring, 369-371 prepared by chemical modification,
375-378
synthetic, 371-375 control of differentiation, 161 development control and, 161 gene derepression and, 160-161, 167 nucleic acid synthesis and, 176 Hormone-receptor complex, 160 House sparrow, LDH from, 409 Human amniotic cells, 253
Human chorionic gonadotropin, 363 Human chorionic somatomammotropin,
363
Human leukemic leukocytes, actinomy
cin D and,178
Human lymphoblastic leukemic cells, 197 Human tumor cells, cordycepin and, 184 Hybridization, specific messenger RNA
and DNA, 213
Hybridized DNA strands, 213
Hydantoic acid, LDH isozymes and, 400 Hydrocortisone
histone synthesis and, 157
tryptophan pyrrolase induction, 187 tyrosine transaminase induction, 187 Hydrolases, 309
Hydroxamic acid, nucleic acid and pro
tein synthesis, 190 Hydroxapatite, 159
2- (<*-Hydroxybenzyl) benzimidazole, anti
viral activity of, 241-243 iV-Hydroxy guanidine, 190 Hydroxylamine, 4, 5
dephosphorylation of NaK-ATPase, 17 DNA fragmentation and, 188
NaK-ATPase, effect on, 29-30 20a-Hydroxysteroid dehydrogenase, 365
i gene, 137
deletion and nonsense mutation in, 147
ICSH, 363
Idiopathic thrombocytopenic purpura, thiopurines and, 128
IgG antibody formation of, 118
response, suppression by 6-mercapto- purine, 118
IgM antibody formation of, 118
response, suppression by 6-mercapto- purine, 118
ilv (ADE) operon, 150 Iminoglycinuria, 101
Immune lysis, antibody response and, 118
Immune reactions antibody responses, 109
cellular immune responses, 109, 124 Immune response
antibody and, 118 enhancement of, 123-124 environmental factors and, 119 genetic factors and, 119
immunosuppressant susceptibility and, 118
thymus dependence of, 118
Immunological injury, resistance to, 119 Immunological studies, LDH isozymes,
416
Immunological tolerance antigen and, 122
lymphoid system and, 122
Immunosuppressant pretreatment, anti
body formation and, 124 Immunosuppressants, 110-114 autoimmune disease and, 129 chronic hepatitis and, 129 differential effectiveness, 118 hemolytic anemia and, 128
immune response susceptibility and, 118
kidney transplant and, 123 lupus nephritis and, 128 lymphoid system damage, 120 polymorphonuclear leukocytes and,
123
protein synthesis and, 120 rheumatoid arthritis, 129 structures of, 112 toxicity and, 110, 119 toxicity of, 129 Immunosuppression
drug administration and, 115 heterologous erythrocytes and, 120 immunological tolerance and, 126 specificity of, 110
Immunosuppressive mechanisms, bio
chemical, 119
Immunosuppressive regimens
immunosuppressant pretreatment and, 124
variables
antigen requirements, 115 antigen strength and, 116-118 drug and dosage, 115
species variability and, 118
timing of drug and antigen, 115-116 ind prophage, λ repressor and, 140 ind~ repressor, 141
Induced mutants, 208 Inflammatory response, 123
adrenal corticoids and, 123 6-mercaptopurine and, 123
I
6-Hydroxyuracil, tobacco mosaic virus and, 295
Hydroxyurea, 188-190
antileukemic activity of, 188 DNA fragmentation and, 188 DNA:RNA ratio and, 189 iV-Hydroxyurethane, 190
Hymenolepis diminuta, proline absorp
tion by, 90 Hyperthyroid, 96
Hypoglycemia, amino acid uptake and, 95
Hypophysectomized rats, insulin in, 95 Hypothalamic hypophysiotropic hor
mones, 367
Hypothalamic releasing factors, 367-368 Hypothalamus, 367
Hypoxanthine, ring spot virus and, 297
SUBJECT I N D E X 487
Influenza virus
actinomycin D and, 252-253, 292 amantadine and, 239
RNA synthesis by, 261
Influenza virus AO (NWS), rifampicin and, 249
Initiation molecules, 162-163
Inorganic salts, virus increase and, 290 Inosine monophosphate dehydrogenase,
mycophenolic acid and, 340 Inotropic effect, 8, 12
Insect-transmitted viruses, 278 Insulin, 366-367
amino acid transport and, 94-95 antilipolytic activity of, 367 histone synthesis and, 157 protein biosynthesis and, 94-95 repression of gluconeogenic enzymes,
155
Insulinlike compounds, 95 Intercellular channels, 87
Interferon, virus production and, 254 Interstitial deletions, 221
Interstitial-cell-stimulating hormone, 363 Inverted transcription, 251
Iodide, NaK-ATPase and, 31
Iodoacetamide, effect on NaK-ATPase, 24-25
4-(Iodoacetamido)salicylic acid, LDH isozymes and, 418-419
Iodoacetate, effect on NaK-ATPase, 24-25
Iodoacetate esters effect on NaK- ATPase, 24-25
5-Iodo-2'-deoxyuridine, 262-265 Ion-conducting antibiotics, 313-319 Ion conduction by antibiotics, 342-343 Ion transport, induction by gramicidins,
319 Ionic strength
DNA polymerase and, 194 LDH isozymes and, 412 Ionizing radiations, 176 Ionophorous antibiotics, 317 IPTG, 138, 145
Iron-chelating antibiotics, 340 Isatin-/3-thiosemicarbazone, 244-246 Isocymarin, 11
Isoleucine, 74
biosynthesis, repression control of, 150 Isoniazid, 306
Isonicotinic acid hydrazide, 93 Isopropylthiogalactoside, 138, 145 Isozymes, 383-425, see also individual
enzymes
application of, 385-386
differential feedback inhibition and, 385
electrical charges and, 384-385 mechanisms for generation of, 397 metabolic significance, 384-388 polymorphism and, 395 tissue-specific patterns, 400 ITP, substrate for NaK-ATPase, 6 ITSC, 244-246
actinomycin D and, 245 IUDR, 262-265
phosphorylation of, 264
J
Jacob-Monod model, 136-137
Κ
K-activated ATPase, pH effect on, 11 K-dependent phosphatase, 4, 8
activation by ATP, 8 diethylstilbestrol, effect of, 36 diisopropylfluorophosphate and, 31 erythrocyte, active center of, 14 hexestrol, effect of, 36
inhibition by ATP and ITP, 6 Vibrio-cholerae inhibitor and, 38 Kanamycin
miscoding and, 334-335 mulberry dwarf disease and, 299 Kanchanomycin, 327
Karrhexis, hydroxyurea and, 189 Kasugamycin, 335
KB cells, RNA synthesis in, 187 Keratitis, IUDR and, 263
α-Ketobutyrate, threonine synthesis and, 150
Ketoisovalerate, leucine biosynthesis and,150
Ketomycin, 333 Kidney
immunological resistance, 119 surgical removal of
renal nitration and, 3 sodium reabsorption, 3 transplantation
azathioprine and, 126 immunosuppressants and, 123 survival percentage, 127-128
L
L cells, 177
L system (exchanging carrier), 81-82 lac operon, 143
constitutive protein production and, 147
lactose and, 139 lac permease, 57
azide, effect of, 62 2,4-dinitrophenol and, 62 formaldehyde and, 58 lac promoter mutant, 145 lac repressor, 137-140, 165
assays, 138
catabolite repressor and, 143 lactose and, 139
molecular weight, 138 lac Repressor-DNA binding
binding constants, 139 cyclic AMP and, 139 ionic strength and, 139
o-nitrophenyl-)3-D-fucoside and, 139 lac system, 136, 137, 141, 142, 167 D-Lactate, 49-50
D-Lactate dehydrogenase, 50
Lactate dehydrogenase, electrical charges and, 385
Lactation, 365
Lactic dehydrogenase A- and B-chain genes of, 155
Lactose
lac repressor and, 139 transport system for, 51 lambda repressor, 140-142, 165 ci gene mutation and, 141
molecular weight, 141 UV irradiation and, 140
Landschutz ascites tumor cells, 193-194 Laodelphax striatellus, 293
Lauryl sulfate, NaK-ATPase, effect on, 36
LDH, 50, 385
LDH-5, X-ray diffraction pattern of, 399 LDH-inhibiting peptides, 386
LDH isozyme activity
experimental conditions and, 392 skeletal muscle, 390
LDH isozymes, 399-419 configuration, 396 genetic origin of, 386
immunological studies on, 416 inhibitors of, 391
ionic strength and, 412 molecular weights, 391-392 N A D and zymogram pattern of,
390-391
patterns in fish, 393-394 pH and, 412
proteolytic degradation of, 386, 390 structure hypothesis of, 392
temperature and, 412 thermal stability of, 413 Leafhoppers, 287
Leakiness, antibiotics and, 312 Lecithinase, 65
Leucine analogs, 99
biosynthesis, repression control of, 150 stimulation of NaK-ATPase, 9 4-Leucine oxytocin, antivasopressin ac-
tivity of, 372
Leucyl-tRNA, repression control and, 150, 166
Leukopenia, renal homograft and, 127 LH, 363
Ligation, enzyme-mediated, 226 Lincomycin, mode of action, 337 Lipid, effect on, amino acid transport
and accumulation, 91-92
Lipid carrier, dephosphorylation of, 309 Lipid-lipid interactions, 65
Lipid membranes antibiotics and, 311-319 disorganization of, 311-313
SUBJECT INDEX 489
Lipid-protein interactions, 65 Lipid-reacting agents
detergents, 64 digitonin, 64 organic solvents, 64 phospholipases, 64
Lipid solubility, antibiotics and, 343 Lipolytic action, epinephrine and, 366 Lipophilic amino acids, 98
Liver regeneration, gene derepression and, 159
Local-lesion method, 278 Lubrol WX, 36
Lucerne mosaic virus, 295 Lupus erythematosus, 128, 129 Lupus nephritis, immunosuppressants
and, 128
Luteotropic action, prolactin, 366 Lymphoblastic leukemic cells,
iV-demethylrifampicin and, 197 Lymphocytes
antibody synthesis and, 118 destruction by immunosuppressants,
125
types of responses, 118
Lymphocytic choriomeningitis virus, 242 methotrexate treatment and, 116 Lymphoid leukemia
antipurines and, 113
folic acid antagonists and, 113 Lymphoid neoplasms, 120 Lymphoid system
damage by immunosuppressants, 120 immunological tolerance and, 122, 125 Lysine
accumulation of, 97 aspartokinase and, 385 histones and, 155
transfer, inhibition by neutral amino acids, 79
transport, neutral amino acids and, 90
Lysogenic induction, mitomycin C and, 192
Lysogeny
cl gene and, 140
regulation by cAMP, 145
Lysophosphatides, insulinlike properties of, 95
Μ Macrolides, 197 Macrophages, 239-240
Macrotetrolides, 316
Magnesium-dependent ATPase, see Mg-ATPase
Magnesium ion, DNA polymerase and, 194
Malate dehydrogenase, electrical charges and, 385
Malate dehydrogenase isozymes, 385, 391, 396
4-(Maleamyl)salicyclic acid, LDH iso
zymes and, 418-419 Maleimide derivatives, 20
NaK-ATPase, effect on, 21 Maltose, 59
operon, 147
transport, uranyl ions and, 61 Marboran, 244
MDH, see Malate dehydrogenase Mealybug cells, DNA replication in, 163 Measles virus, actinomycin D and, 254 Mediated diffusion, temperature and,
98^-99
Melanocyte-stimulating hormones, 373 Melanotropins, 362
release inhibiting factor, 363 Melibiose, 210
Melinacidin, 342 Membrane
configuration, cardioactive steroids and, 13
leakiness, 65 lipid
antibiotics and, 311-319, 343 composition, temperature and, 98 precipitation of, 99
nonelectrolyte permeability of, 75 permeability changes, 72
replication point and, 162 structure, myxin and, 328 translocation, 50
Membrane-active agents, 64 Mendelian recessives, 100
Mengovirus, actinomycin D and, 251, 253
Meralluride, 21
β-Mercaptoethanol, 417 2-Mercaptoethanol
LDH isozymes and, 400 sarkomycin and, 193 Mercaptomerin, 21 6-Mercaptopurine, 111
autoimmune disease and, 128 differential effectiveness of, 118 DNA synthesis and, 120 effects of, 113
immunological paralysis and, 122 inflammatory response and, 123 secondary response to antigen and, 117 Mercurial diuretics, 20
Mercuric chloride, 60 Mercury ions, toxicity of, 35
Mesotocin, water reabsorption and, 370 Messenger RNA synthesis, 245
actinomycin and, 178
Metamorphosis, thyrotropin induced, 366 Methacycline, 299
Methanesulfonylchloride, effect on NaK-ATPase, 32
Methionine
aspartokinase and, 385 transfer, sodium ion and, 82 transport
anaerobic, stimulation of, 98 pH and, 88
sulfur starvation and, 92 Methisason, 244
smallpox and, 237 Methotrexate, 111, 123
autoimmune thyroiditis and, 128 differential effectiveness of, 118 dihydrofolate reductase and, 120 inflammatory response and, 123 skin graft and, 116
toxicity of, 113, 129 4-Methoxyphloretin, 54 Methyl acetate, 243
iV-Methylacetohydroxamic acid, 190 α-Methyl-l-adamantanmethylamine hy
drochloride, 241 iV-Methyl-AIB, 80
iV-Methylated amino acids, transport of, 74-75
iV-Methylated rifampicin, RNA-depen
dent D N A polymerase and, 250
Methyl formate, 243
2-Methyl gluconate, transient repression and, 144
3-O-Methylglucose accumulation, so
dium ion and, 85
3-Methyl-D-glucose, phenolphthalein and uptake of, 37
α-Methylhistidine, histidine biosynthesis and, 149
N-Methylhydroxylamine, 30, 190 iV-Methylhydroxyurea, 190 iV-Methylisatin-jS-4', 4'-dibutylthio-
semicarbazone, 246 N-Methyl-ITSC, 244
Methyloxytocine, uterotonic properties of, 371
3-(4-Methylpiperazinyliminomethyl) rifamycin SV, 246
Methylprednisolone, 3
6-Methyl-2-thiouracil, tobacco mosaic virus and, 295
4-Methyltryptophan, 101
2-O-Methyltyrosine oxytocin, 371 Methylurea, LDH isozymes and, 400 Methymycin, effect on NaK-ATPase, 28 Mg-activated RNA polymerase,
α-amanitin and, 181 Mg-ATPase
beryllium ions, effect of, 32
chlorinated hydrocarbons, effect of, 33 detergents, effect of, 36
fluoride and, 31
human red blood cell membranes, 20 sulfhydryl reagents, effect of, 20 turtle bladder mucosal cells, micro
somes of, 20 urea, effect of, 37-38
Micrococcus luteus, endonuclease of, 221 Micrococcus lysodeikticus, RNA poly
merase from, 184 Microvilli, 87 Mineralocorticosteroids
sodium transport and, 160 transcription and, 160
Minus-strand RNA synthesis, actinomy
cin D and, 253
Miracil D, intercalation with DNA, 176 Mitchell chemiosmotic hypothesis, 62
