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Number Title of presentation Slide Title of slide
1. Basic principles 1. Cover page
2. Basic principles (Title) 3. Aim of the course 4. Course contents
5. What is tissue engineering?
6. Basic principles of tissue engineering 7. 2D tissue cultures
8. 3D tissue cultures 9. Engineering tissues 10. Bioreactor
11. Potential uses of cell and tissue replacement therapy
12. Cells in tissue engineering
2. Stem cells (1) 1. Cover page
2. Stem cells (1) (Title) 3. Stem cells
4. Stem cell types
5. Types of stem cell replications I 6. Types of stem cell replications II 7. Sources of stem sells
8. Epiblast stem cells I 9. Epiblast stem cells II
10. Location of multipotent adult somatic stem cells was identified
11. Adult (somatic) stem cells (ASC) 12. The adult stem cell niche 13. Marrow stem cells (MSCs)
14. Hematopoietic stem cells (HSCs) I 15. Haematopoietic stem cells (HSCs) II 16. Hematopoietic stem cells (HSCs) III 17. Mesenchymal stem cells (MSC) I 18. Mesenchymal stem cells (MSC) II 19. Endothelial progenitor cells (EPC) 20. Bone marrow progenitor cells
21 Ontogeny of tissue lineages in bone marrow 22. Functional interdependency of bone marrow stem
cells
3. Stem cells (2) 1. Cover page
2. Stem cells (2) (Title) 3. Cord blood stem cells
4. Cord blood stem cells and fetal stem cells 5. Cryopreservation
6. Cord blood processing
7. Cord blood processing and cryopreservation 8. Cord blood banking
9. Pluripotenciy of cord blood stem cells 10. Stem cell population in cord blood 11. Disorders treatable with cord blood I 12. Disorders treatable with cord blood II
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13. Fat stem cells
14. Types of adipose tissues 15. Isolation procedures
16. Immunophenotype of ASCs - Positive markers 17. Immunophenotype of ASCs - Negative markers 18. Cytokine profile of ASCs
19. Immunogenecity of ASCs 20. Differentiation potential of ASCs 21. Differentiation into adipocytes 22. Differentiation into cardiac myocytes 23. Differentiation into chondrocytes 24. Differentiation into osteocytes 25. Differentiation into skeletal myocytes 26. Differentiation into neuronal cells
27. Differentiation into endodermal and ectodermal lineages
28. Differentiation into endothelial and smooth muscle cells
29. Hematopoietic support
4. Stem cells (3) 1. Cover page
2. Stem cells (3) (Title)
3. Application of ESCs and ASCs
4. Genetic engineering and gene delivery using ASCs 5. Approaches and methods for controlling stem cell
growth and differentiation 6. Reprogramming
7. Differentiation of cells I.
8. Differentiation of cells II.
9. Mature, organ specific primary cells I.
10. Mature, organ specific primary cells II.
11. Differentiation of epidermis
12. Mature tissue specific cells in tissue engineering 13. Regulatory issues I. Cells
14. Regulatory issues II. Animals
15. Regulatory issues III. Human embryonic stem cells 16. Regenerative medicine
17. Organ failure
5. Bioreactors (1) 1. Cover page
2. Bioreactors (1) (Title) 3. Static cell cultures
4 Problems concerning static cell cultures 5. Bioreactors: dynamic cell environment
6. Mass transport challenges in 3D tissue cultures 7. Shear forces in dynamic fluids
8. Shear stress in bioreactors
9. Cell distribution in dynamic environment 10. Bioreactor design requirements
11. Bioreactor design requirements 12. Structure of an industrial bioreactor 13. Spinner flask bioreactors
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14. Rotating wall bioreactors I.
15. Rotating wall bioreactors II.
16. Compression bioreactors I.
17. Compression bioreactors II.
6. Bioreactors (2) 1. Cover page
2. Bioreactors (2) (Title) 3. Strain bioreactors
4. Flow perfusion bioreactors I.
5. Flow perfusion bioreactors II.
6. Cartilage: tissue features and injury repair 7. Compression bioreactors for cartillage TE I.
8. Compression bioreactors for cartillage TE II.
9. Tissue Engineering in bone repair
10. Flow perfusion bioreactors in bone engineering 11. Two chamber bioreactor
12. Current achievements in bioreactor design 13. Limitations of current bioreactors
7. Biomaterials (1) 1. Cover page
2. Biomaterials (1) (Title)
3. Biomaterials used in tissue engineering 4. Natural biomaterials I.
5. Natural biomaterials II.
6. Collagen I.
7. Collagen II.
8. Fibrin 9. Silk I.
10. Silk II.
11. Polysaccharide-based biomaterials 12. Agarose
13. Alginate
14. Hyaluronan (Hyaluronic acid) 15. Chitosan
16. Chitosan in bone TE
8. Biomaterials (2) 1. Cover page
2. Biomaterials (2) (Title) 3. Synthetic biomaterials I.
4. Synthetic biomaterials II.
5. Poly (lactic-co-glycolic acid), PLGA 6. Poly-(ethylene glycol), PEG 7. Peptide-based biomaterials 8. Ceramic-based biomaterials 9. Metals
9. Scaffold fabrication 1. Cover page
2. Scaffold fabrication (Title) 3. Basic criteria for scaffolds I.
4 Basic criteria for scaffolds II.
5. Importance of scaffold characteristics
6. Solvent casting and particulate leaching (SCPL) I.
7. Solvent casting and particulate leaching (SCPL) II.
8. Phase separation methods
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9. Advanced techniques - Gas foaming 10. Electrospinning I.
11. Electrospinning II.
12. Advanced techniques - Fiber mesh 13. Fiber mesh
14. Advanced techniques - Self assembly 15. Design of peptide ampholites
16. Advanced techniques - Rapid prototyping
17. Advanced techniques - Fused deposition modeling (FDM)
18. Advanced techniques - Selective laser sintering (SLS)
19. Selective laser sintering (SLS)
10. Biocompatibility 1. Cover page
2. Biocompatibility (Title) 3. Biocompatibility - Definition 4. Biocompatibility - Recent views 5. Biocompatibility of biomaterials 6. Biocompatibility - Terminology 7. Biocompatibility testing
8. Complications from incompatibility 9. Normal wound healing
10. Foreign Body Reaction I.
11. Foreign Body Reaction II.
12. Biomaterials 13. Bioinert materials 14. Silicone derivates 15. Biocompatible metals 16. Hydroxyiapatite ceramics
17. Poly--hydroxy-acids: bioabsorbable polymers 18. Degradation of poly--hydroxy-acids
19. Biodegradation of poly--hydroxy-acids 20. Application of poli-a-hydroxy-acids 21. Poly-(Glycolic Acid), PGA
22. Poly-(Lactic Acid), PLA and PGA co-polymers 23. Biodegradation of polylactides
24. Poly-(caprono-lactone), PCL 25. Polymer erosion
26. Types of degradation in biomaterials 27. Degradation I.
28. Degradation II.
29. Degradation III.
11. Cell-Scaffold interaction 1. Cover page
2. Cell-Scaffold interaction (Title) 3. Scaffolds I.
4. Scaffolds II.
5. Cell line cells nestling on a scaffold 6. Matrigel®
7. Primary SAEC (Small Airway Epithelial Cells) in matrigel
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12. Biofactors 1. Cover page
2. Biofactors (Title) 3 Biofactors
4 Main growth factors (GFs) used in tissue engineering
5. VEGF 6 TGF-
7. BMP
8 FGF
9. PDGF
10. Delivery of growth factors
13. Controlled release 1. Cover page
2. Controlled release (Title)
3. Controlled drug delivery from scaffolds 4. Ideal scaffold
5. ECM mimicry as a guide for scaffold design 6. Growth factors and the ECM
7. Mimic the function of ECM 8. Interspersed signals 9. Immobilized signals 10. Signal delivery from cells
11. Protein delivery systems (DS) in TE 12. Non-biodegradable systems 13. Biodegradable systems
14. Controlled release profiles in biodegradable systems
15. On-off drug delivery systems
16. Programmed and triggered delivery systems 17. Inclusion of drug molecules into scaffolds 18. Inclusion of bioactive proteins into scaffolds 19. VEGF supports TE tissue vascularization 20. Support of tissue differentiation with bioactive
proteins
21. Experimental results with controlled drug delivery scaffolds – VEGF
22. Clinical results with controlled drug delivery scaffolds – BMP-2
14. Biosensors 1. Cover page
2. Biosensors (Title) 3. Definition
4. Concept of an implantable glucose sensor 5. SEM morphology of the dexamethasone-loaded
PLGA microspheres
6. Model of biosensor-tissue interactions 7. The “intelligent” system
8. Development of reliable glucose biosensors require
15. Aggregation cultures 1. Cover page
2. Aggregation cultures (Title) 3. Aggregate cultures
4. Principals of aggregate cultures
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5. Cell adhesion
6. Methods of cell aggregation 7. Gravity cultures
8. Suspension aggregate cultures 9. Aggregation in rotation culture 10. Bioreactors and cell aggregation 11. Cell type aggregates using bioreactors 12. Application of the cell aggregates 13. Microgravity culture (hanging drop) I.
14. Microgravity culture (hanging drop) II.
15. Microwells for uniform embryoid body culture and control of cell-cell contact
16. Aggregation on low adherence surfaces 17. Natural cell aggregation
18. Synthetic cell aggregation I.
19. Synthetic cell aggregation II.
20. Biotinylated cell cross-linking 21. Chemical modification of surfaces 22. Chitosan
23. Modified PEG
24. Lactone modified eudragit 25. PLGA nanospheres 26. Lectins and derivatives I.
27. Lectins and derivatives II.
28. Types of N-glycans recognised by PHA 29. Cell aggregation on scaffolds
30. Nanostructured scaffolds
31. Nanomaterials for aggregate cultures
16. Tissue printing 1. Cover page
2. Tissue printing (Title)
3. Main principles of tissue printing 4. Cell clusters fuse into micro-tissues
5 Cell clusters fuse into micro-tissue sapes 6. Organ printing
7. The first tissue printer
8. Mature, organ specific primary cells I.
9. Mature, organ specific primary cells II.
10. Mature tissue specific cells in tissue engineering 11. Generation of blood vessels
12. Application of blood vessels
17. Tissue repair (1) 1. Cover page
2. Tissue repair (1) (Title) 3. Stem cells in the bone marrow
4. Therapeutic indications for stem cell therapy 5. Embryonic stem cells
6. Germ stem cells
7. Hemapoetic Cell Transplantation (HCT) 8. Hematopoetic stem cells
9. General principles of stem cell therapy 10. Bone repair with stem cells
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11. Cartilage regeneration
18. Tissue repair (2) 1. Cover page
2. Tissue repair (2) (Title) 3. Liver repair
4. Causes of liver failure 5. Liver transplantation
6. Potential in cellular therapy of liver failure 7. Using stem cells for liver regeneration
8 HSC and liver regeneration 9. MSC and liver regeneration
10. Cultured HSC differentiate into hepatocytes 11. Human trials
12. Injury-induced differentiation of BMSC (animal models)
13. Conclusions
19. Tissue repair (3) 1. Cover page
2. Tissue repair (3) (Title) 3. Heart failure
4. Heart regenerative therapies 5. Left ventricle assist device (LVAD) 6. Ventricular assist devices
7. Bone marrow cells in cardiac repair 8 Cellular therapies in cardiac repair I.
9. Cellular therapies in cardiac repair II.
10. Cellular therapies in cardiac repair III.
11. Cellular therapies in cardiac repair IV.
12. Cellular therapy of cardiac muscle 13. Skeletal myoblasts
14. Embryonic stem cells 15. Tissue engineering in tooth
regeneration/replacement 16. Tooth development
17. Dental pulp stem cells (DPSC) 18. Differentiation capacity of DPSC 19. Bioenginnered tooth concepts 20. De novo tooth engineering I.
21. De novo tooth engineering II.
20. Tissue repair (4) 1. Cover page
2. Tissue repair (4) (Title)
3. Major causes of urogenital injuries
4. Repair possibilities of the urogenital organs 5. Obtaining cells for tissue regeneration
6. Biomaterials for genitourinary reconstruction I.
7. Biomaterials for genitourinary reconstruction II.
8. Uroepithel – unique features 9. Urethra reconstruction I.
10. Urethra reconstruction II.
11. Urethra reconstruction III.
12. Bladder reconstruction I.
13. Bladder reconstruction II.
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14. Bladder reconstruction III.
15. Ureter reconstruction 16. Kidney replacement therapy 17. Dialysis
18. Kidney transplantation 19. Tissue engineered kidney 20. Bioartificial kidney
21. Tissue engineered kidney
22. In vitro engineered murine kidney
21. Commercial products (1) 1. Cover page
2. Commercial products (1) (Title) 3. Organ failure
4 Regenerative medicine
5. Commercialization of tissue engineering 6. Cardiovascular diseases
7. Artificial heart valves 8. Biological heart valves
9. Tissue engineered heart valves 10. Replacement of blood vessels 11. Vascular tissue engineering 12. Developments in vascular TE 13. Vascular grafts
14. Vascular tissue engineering 15. Tissue engineered blood vessel 16. TEBV production
17. Cartilage injury and regeneration 18. Challenges for cartilage TE
19. Autologous chondrocyte implantation (ACI) I.
20. Autologous chondrocyte implantation (ACI) II.
21. Autologous chondrocyte implantation (ACI) III.
22. Commercial products for ACI 23. Matrix-induced ACI (MACI)
22. Commercial products (2) 1. Cover page
2. Commercial products (2) (Title) 3. Bioartificial liver Assist Device 4. Liver dialysis
5. Bioartificial liver 6. ELAD® bioartificial liver 7. Cell-free ELAD: MARS 8. Skin grafting and replacement 9. Structure of the skin
10. Purpose of skin grafting 11. Split-thickness grafts 12. Autologous Skin grafts 13. Integra® skin replacement
14. CEA – Cultured Epithelial Allograft
23. Clinical trials (1) 1. Cover page
2. Clinical trials (1) (Title) 3. Definition of a clinical trial 4. Types of clinical trials
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5. Participants in a clinical trial
6. Benefits of taking part in a clinical trial 7. Risks of clinical trials
8. Process of clinical trials 9. Phases of clinical trials
10. Stem cell therapy and clinical trials
11. Stages to reach a marketable cell-based therapeutic product
24. Clinical trials (2) 1. Cover page
2. Clinical trials (2) (Title)
3. Clinical trials for cellular therapy of liver failure 4. Liver dialysis
5. Bioartificial liver 6. ELAD® bioartificial liver 7. Cell-free ELAD: MARS 8. Skin grafting and replacement 9. Structure of the skin
10. Purpose of skin grafting 11. Skin grafts
12. Autologous skin grafts
13. CEA – Cultured Epithelial Allograft I.
14. CEA – Cultured Epithelial Allograft II.
15. Tissue engineered skin methodology
16. Tissue engineered skin products available for clinical use
25. Clinical trials (3) 1. Cover page
2. Clinical trials (3) (Title)
3. Tissue engineered blood vessel 4. TEBV production
5. Myocardial infaction 6. Urethra and bladder 7. Cartilage
8. Lung
9. Trachea - bronchi - lungs
10. Lung diseases benefiting from cell based therapies 11. Strategies for cell-based therapies for the lung 12. Complexity of lung structures during development 26. Research application and
drug testing
1. Cover page
2. Research application and drug testing (Title) 3. Research applications
4. Thymus reaggregation
5. Thymocyte development in the reaggregated thymus
6. Salivary gland aggregate 7. Tissue specific changes 8. Drug testing
9. Hepatotoxicity testing 10. Lung model for drug testing
27. Ethical issues 1. Cover page
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2. Ethical issues (Title) 3. No ethical issues raised 4. More ethical questions 5. Regulation in Europe
6. The most relevant documents 7. Ruling in 2011
8. USA
28. Economic significance 1. Cover page
2. Economic significance (Title) 3. Biotechnology
4. Significance in the US 5. Significance in the EU 6. Big Pharma
