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Essential and toxic microelements in grains of Fusarium- infected maize and wheat genotypes:
ICP-MS analysis
Zsuzsanna Kolbert, Attila Ördög, Ágnes Vashegyi, László Erdei
Materials and methods
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Sample preparation :
• 500 – 1000 mg from each sample
• microwave digestion in nitric acid and hydrogen peroxide
• dilution to 200 - 400 X
Measurement:
• Microelement analysis (Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Pb) by ICP- MS device
Plant material :
• 2 maize genotypes (two sampling times)
• 13 wheat genotypes
Infection :
• Fusarium graminearum
• Fusarium culmorum
• Fusarium verticillioides
The advantages of an ICP-MS
• More than 1000 samples in one week (up to 250 samples/day)
• More than 15 000 data from the analysis
• Lot of information in a few seconds (less than a second per element)
• User-friendly software (PlasmaLab), which makes the data analysis easier and faster
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Microelement contents in the two maize genotypes
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GK-Sarolta GK-Boglár
Mn content in maize genotypes
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Two countries, one goal, joint success!
Fe content in maize genotypes
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Co content in maize genotypes
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Ni content in maize genotypes
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Cu content in maize genotypes
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Zn content in maize genotypes
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As content in maize genotypes
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Se content in maize genotypes
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Mo content in maize genotypes
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Cd content in maize genotypes
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Pb content in maize genotypes
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Microelement contents in 13 different wheat genotypes
• Manastur - TM
• Sanandrei - TM
• Faget-TM
• Pietroasa- TM
• Lovrin -TM
• Aldesti -TM
• Dudestii Vechi -TM
• Horea - Ar
• Siria - Ar
• Recas - Ar
• Semlac - Ar
• Paulis - Ar
• Barzava - Ar
Mn content in different wheat genotypes
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Fe content in different wheat genotypes
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Co content in different wheat genotypes
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Ni content in different wheat genotypes
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Cu content in different wheat genotypes
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Zn content in different wheat genotypes
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As content in different wheat genotypes
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Se content in different wheat genotypes
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Two countries, one goal, joint success!
Mo content in different wheat genotypes
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Cd content in different wheat genotypes
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Pb content in different wheat genotypes
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Pb content in different wheat genotypes
Summary
Maize genotypes:
• Infections by all Fusarium strains increased the concentrations of microelements (Mn, Fe, Ni, Cu) and toxic metals (Cd, Pb) in the grains
Wheat genotypes:
• Mn and Ni concentration were high in Barzava-Ar
• Fe, Co, As content were higher in the chaff compared to grains in all genotypes
• In the chaff of Horea-Ar high Pb and As concentrations were measured
protection for the grain
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Two countries, one goal, joint success!
