Element content of Fusarium-infected wheat genotypes
Szeged – Timişoara axis for the safe food and feed SZETISA1
HURO/0901/147/2.2.2
Reporting period: 01.06. 2011 – 30.09. 2011
2nd Progress Report Meeting Szeged, 22-23 September, 2011
Erdei László, Pető Andrea, Csiszár Jolán
Department of Plant Biology University of Szeged
The wheat quality and consumer safety is threatened by Fusarium Head Blight (FHB) caused by i.e. Fusarium culmorum, F.
graminearum and F. avenaceum.
Diseased spikelets exhibit symptoms of premature bleaching shortly after infection.
Hypothesis: Fusarium infection influences
physiological, biochemical and membrane-transport
characteristics of the plant resulting in altered mineral
composition of the grain.
Visible effects of Fusarium sp.
on wheat
I. Gathering wheat ears and flag leaves
17. 06. 2010. Kecskéstelep, Cereal Research Nonprofit Kht.
1. GK Mini Manó/Nobeokabozu wheat population (Prof. Mesterházy Ákos )
Genetic mapping population with 350 members.
The Fusarium resistance is a complex, quantitative character.
The aim is to develop lines with several
resistance genes.
After crossing, the
progenies will be inbred to increase the
homogenity
(recombinant inbred lines)
1. GK Mini Manó/Nobeokabozu genetic mapping population
(Prof. Mesterházy Ákos )
Nobeokabozu: FHB resistant line
A recombinant line from China: ‘WSY’, Wangshubai/Sumai3/Yangangfanzhou
GK Mini Manó
Arthur 71 / Sava // Rusalka / NS 171.2 /3/ F 30.74
Resistant line to e.g. leaf rust
I. Gathering wheat ears and flag leaves
18. 05. 2011. Kecskéstelep, Cereal Research Nonprofit Kht.
1. GK Mini Manó/Nobeokabozu wheat population (Prof. Mesterházy Ákos )
The aim is to develop lines with
several Fusarium resistance genes.
After crossing, the progenies will be inbred to increase the homogenity (recombinant inbred lines)
I. Sampling before inoculation 18. 05. 2011.
• 3-3 ears with stem were taken for ROS and NO detections
The fluorescens investigations were done as soon as it was possible
Timetable - Planned chronology
• 1rst measurements and sampling: 05.18.
• Inoculation: 05. 26.
with Fusarium culmorum (Fc12551) and F. graminearum (Fg46.06 ) isolates
• The bags were removed after 2 - 3 days
• II. sampling after the symptomes will be developed (ca. after 3 weeks)
• III. seeds will be harvested after another 3 weeks
Lines inoculated with two Fusarium species:
Fusarium culmorum Fusarium graminearum
Line 561 – very resistant against Fusarium (Sumai3 from China) 1/a – inoculated with Fusarium culmorum
2/a – inoculated with Fusarium graminearum
Line 474 – earlier Fusarium resistant (alias 484), high Mn level in the ear 1/a – inoculated with Fusarium culmorum
2/a – inoculated with Fusarium graminearum
Line 501– earlier Fusarium resistant (alias 515), low Mn level in the ear 1/a – inoculated with Fusarium culmorum
2/a – inoculated with Fusarium graminearum
Line 483 – earlier Fusarium sensitive (alias 493), high Mn level in the ear 1/a – inoculated with Fusarium culmorum
2/a – inoculated with Fusarium graminearum
Line 488 – earlier Fusarium sensitive (alias 500), low Mn level in the ear 1/a – inoculated with Fusarium culmorum
2/a – inoculated with Fusarium graminearum
Accumulation of Cu, Mn, Fe, Zn mikroelements and K, Ca Mg macroelements
in different plant parts
Comparison of element concentrations in flag leaf, culm, green ear , and in the ripe ear in grains and husks
Wheat lines:
Sumai-3: resistant to Fusarium („line 561”) Lines regarded as resistant: „474”, „501”
Lines regarded as sensitive: „483”, „488”
Resistant Sensitive High Mn
Low Mn
General gradient of Cu:
from flag leaf to chaff
Resistant
Sensitive High Mn
General gradient of Mn:
from flag leaf to chaff
Resistant
Sensitive High Mn
Low Mn
General gradient of Fe from flag leaf to chaff
Resistant
Sensitive High Mn
General gradient of Zn:
accumulation in grain
Resistant
Sensitive High Mn
Low Mn
General gradient of K:
accumulation in culm
Resistant
Sensitive High Mn
General gradient of Ca:
accumulation in chaff
Resistant
Sensitive High Mn
Low Mn General gradient of Mg:
from flag leaf to chaff
Accumulation of Cu, Mn, Fe, Zn mikroelements and K, Ca Mg macroelements
in different plant parts:
Comparison of wheat lines and their responses to Fusarium inoculation
To be observed:
-direction of transport (e.g. from schaff to grain, Cu, Zn)
Conclusion
Gradient s of element concentrations are different from flag leaf to the grain usually decerasing (Cu, Mn, Fe)
but increasing for Zn
only xylem transport, Ca (it is known fact),
resulting in accumulation in the chaff accumulation in the tissue of the culm (K).
Direction of transport (e.g. from schaff to grain, Cu, Zn) Effects of inoculation, maybe artefact:
