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(1)Supplement 3A Seasonal Mann-Kendall and Mann-Kendall trend analysis of major environmental variables in the middle Danube section, Göd (N-Budapest, Hungary) between 1979 and 2012

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Supplement 3A Seasonal Mann-Kendall and Mann-Kendall trend analysis of major environmental variables in the middle Danube section, Göd (N-Budapest, Hungary) between 1979 and 2012. Tau and significance levels (in brackets) are given, as well as bootstrap confidence interval calculations (BCICs) for the Mann-Kendall tests based on 10,000 bootstrap replicates at 99% CI (in second line). Significant trends are bold and italic.

Parameter GLOBAL

(Seasonal M-K)

WINTER

(Mann-K) SPRING

(Mann-K) SUMMER

(Mann-K) AUTUMN (Mann-K) Water temperature (°C) 0.25 (***) 0.11 (n.s.)

0.03, 0.42

0.11 (n.s.) 0.10, 0.32

0.30 (***) 0.38, 0.82

0.07 (n.s.) 0.04, 0.25 Total suspended solids (mgL-1) -0.35 (***) -0.31 (***)

0.89, -0.36 -0.32 (***)

-0.90, -0.39 -0.31 (***)

-0.87, -0.37 -0.32 (***) -0.87, -0.41 Ammonium-N (mgN L-1) -0.66 (***) -0.70 (***)

-1.77, -1.02 -0.62 (***)

-1.56, -0.91 -0.56 (***)

-1.43, -0.78 -0.65 (***) -1.62, -0.96 Nitrite-N (mgN L-1) -0.58 (***) -0.43 (***)

-1.15, -0.56 -0.58 (***)

-1.50, -0.83 -0.55 (***)

-1.42, -0.77 -0.61 (***) -1.54, -0.88 Nitrate-N (mgN L-1) -0.20 (***) -0.02 (n.s.)

-0.28, 0.19

-0.12 (n.s.) -0.40, -0.08

-0.22 (**) -0.65, -0.21

-0.21 (**) -0.61, -0.22 Orthophosphate-P (µgP L-1) -0.57 (***) -0.63 (***)

-1.62, -0.88 -0.55 (***)

-1.43, -0.78 -0.41 (***)

-1.13, -0.50 -0.59 (***) -1.52, -0.85

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Supplement 3B Seasonal Mann-Kendall and Mann-Kendall trend analysis of the relative abundance of phytoplankton functional traits in the middle Danube section, Göd (N-Budapest, Hungary) between 1979 and 2012. Tau and p values (in brackets) are given, as well as bootstrap confidence interval calculations for the Mann-Kendall tests (in the second line) based on 10,000 bootstrap replicates at 99% CI. Significant trends are bold and italic. For the abbreviation of functional traits, see Table 1 in the article.

FTs GLOBAL (Seasonal M-K)

WINTER (Mann-K)

SPRING (Mann-K)

SUMMER (Mann-K)

AUTUMN (Mann-K)

S 0.10 (**) -0.02 (n.s.)

-0.19, 0.13

0.17 (*) 0.16, 0.51

0.08 (n.s.) -0.07, 0.40

0.10 (n.s.) -0.02, 0.42 M -0.12 (***) -0.05 (n.s.)

-0.27, 0.06

-0.25 (***) -0.73, -0.28

-0.0269 (n.s.) -0.29, 0.19

-0.133 (*) -0.49, -0.04

L 0.04 (n.s.) 0.08 (n.s.)

-0.05, 0.36

0.23 (***) 0.20, 0.71

-0.17 (*) -0.58, -0.09

0.08 (n.s.) -0.06, 0.36

XL -0.08 (*) -0.18 (*)

-0.56, -0.14

0.04 (n.s.) -0.11, 0.28

-0.07 (n.s.) -0.33, 0.06

-0.12 (n.s.) -0.44, -0.04

GAL 0.21 (***) 0.19 (**)

0.18, 0.59

0.38 (***) 0.49, 1.02

-0.00 (n.s.) -0.22, 0.18

0.212 (**) 0.23, 0.62

FLA 0.36 (***) 0.30 (***)

0.37, 0.85

0.32 (***) 0.35, 0.91

0.29 (***) 0.35, 0.82

0.51 (***) 0.72, 1.30 SIN -0.19 (***) 0.06 (n.s.)

-0.07, 0.30

-0.25 (***) -0.70, -0.31

-0.21 (**) -0.59, -0.23

-0.11 (n.s.) -0.40, -0.07

COL 0.01 (n.s.) -0.07 (n.s.)

-0.33, 0.06

0.19 (**) 0.21, 0.55

-0.13 (n.s.) -0.49, -0.02

-0.03 (n.s.) -0.25, 0.13 FIL 0.21 (***) -0.03 (n.s.)

-0.24, 0.12

0.36 (***) 0.48, 0.95

0.23 (***) 0.24, 0.69

0.08 (n.s.) -0.03, 0.34

SIR -0.24 (***) -0.16 (*)

-0.51, -0.12

-0.31 (***) -0.87, -0.39

-0.05 (n.s.) -0.31, 0.12

-0.34 (***) -0.92, -0.43

VAC 0.15 (***) 0.09 (n.s.)

-0.05, 0.41

0.17 (*) 0.14, 0.54

0.136 (n.s.) 0.05, 0.49

0.0878 (n.s.) -0.05, 0.40

N2R 0.02 (n.s.) 0.02 (n.s.)

-0.18, 0.24

0.16 (*) 0.11, 0.52

-0.03 (n.s.) -0.24, 0.13

-0.08 (n.s.) -0.32, 0.02

CHB -0.00 (n.s.) 0.00 (n.s.)

-0.19, 0.21

0.18 (**) 0.19, 0.52

-0.19 (**) -0.62, -0.15

-0.05 (n.s.) -0.32, 0.12

CHC -0.02 (n.s.) 0.04 (n.s.)

-0.12, 0.28

-0.188 (**) -0.54, -0.21

0.134 (*) 0.03, 0.51

0.01 (n.s.) -0.19, 0.23

PYC 0.41 (***) 0.20 (**)

0.20, 0.58

0.37 (***) 0.44, 1.02

0.45 (***) 0.61, 1.20

0.51 (***) 0.72, 1.30

BEN 0.11 (**) 0.05 (n.s.)

-0.11, 0.31

0.24 (***) 0.28, 0.68

0.00 (n.s.) -0.22, 0.23

0.17 (*) 0.14, 0.53 PLA -0.11 (**) -0.05 (n.s.)

-0.32, 0.11

-0.24 (***) -0.69, -0.28

-0.00 (n.s.) -0.23, 0.22

-0.17 (*) -0.53, -0.14

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Supplement 3C Seasonal Mann-Kendall and Mann-Kendall trend analysis of the relative abundance of phytoplankton functional groups (FGs) sensu Reynolds in the middle Danube section, Göd (N-Budapest, Hungary) between 1979 and 2012. Tau and p values (in brackets) are given, as well as bootstrap confidence interval calculations for the Mann-Kendall tests (in the second line) based on 10,000 bootstrap replicates at 99% CI. Significant trends are bold and italic. For the abbreviation of FGs, see Reynolds et al. (2002), Borics et al. (2007), and Padisák, Crossetti &

Naselli-Flores (2009).

FGs GLOBAL (Seasonal M-K)

WINTER (Mann-K)

SPRING (Mann-K)

SUMMER (Mann-K)

AUTUMN (Mann-K) A -0.13 (**) -0.14 (n.s.)

-0.50, -0.04

0.07 (n.s.) -0.05, 0.35

-0.21 (**) -0.66, -0.15

-0.18 (*) -0.60, -0.13 B -0.01 (n.s.) 0.0681 (n.s.)

-0.12, 0.40

0.15 (*) 0.06, 0.54

-0.01 (n.s.) -0.25, 0.22

-0.26 (***) -0.80, -0.23 C -0.11 (**) -0.06 (n.s.)

-0.36, 0.11

-0.09 (n.s.) -0.37, 0.01

-0.07 (n.s.) -0.367, 0.08

-0.24 (***) -0.71, -0.23 D -0.23 (***) -0.17 (*)

-0.53, -0.13

-0.31 (***) -0.87, -0.36

-0.07 (n.s.) -0.35, 0.07

-0.33 (***) -0.91, -0.41

E 0.16 (***) 0.37 (***)

0.44, 1.02

0.25 (***) 0.27, 0.73

-0.12 (n.s.) -0.45, -0.01

0.13 (n.s.) 0.04, 0.49 F -0.10 (**) -0.13 (n.s.)

-0.50, -0.04

0.07 (n.s.) -0.02, 0.30

-0.24 (***) -0.75, -0.22

-0.11 (n.s.) -0.44, 0.01

G -0.07 (n.s.) 0.17 (*)

0.13, 0.54

0.08 (n.s.) 0.00, 0.34

-0.23 (***) -0.69, -0.23

-0.16 (*) -0.56, -0.09 H1 0.02 (n.s.) 0.02 (n.s.)

-0.18, 0.24

0.18 (*) 0.14, 0.57

-0.04 (n.s.) -0.26, 0.11

-0.09 (n.s.) -0.36, -0.01 J 0.02 (n.s.) -0.01 (n.s.)

-0.18, 0.14

0.23 (***) 0.28, 0.62

-0.16 (*) -0.56, -0.09

-0.09 (n.s.) -0.39, 0.02 K 0.07 (n.s.) -0.16 (n.s.)

-1.80, 0.59

0.26 (**) -0.29, 1.19

0.12 (n.s.) -0.09, 0.58

-0.05 (n.s.) -0.44, 0.22 L0 0.03 (n.s.) -0.07 (n.s.)

-0.38, 0.11

0.13 (n.s.) 0.03, 0.48

0.18 (**) 0.11, 0.61

-0.09 (n.s.) -0.39, 0.01

M 0.19 (***) 0.13 (n.s.)

-0.06, 0.56

0.09 (n.s.) -0.41, 0.69

0.23 (**) 0.18, 0.73

0.14 (n.s.) 0.01, 0.55

P 0.27 (***) 0.26 (***)

0.21, 0.81

0.46 (***) 0.58, 1.24

0.16 (*) 0.08, 0.53

0.13 (n.s.) 0.06, 0.46 Q 0.11 (n.s.) -0.04 (n.s.)

-1.68, 0.81

0.02 (n.s.) -1.56, 0.92

0.08 (n.s.) -0.20, 0.49

0.18 (*) -0.53, 1.06 S1 0.12 (***) 0.04 (n.s.)

-0.14, 0.31

0.25 (***) 0.28, 0.73

-0.01 (n.s.) -0.19, 0.17

0.16 (*) 0.13, 0.51 S2 -0.20 (***) -0.28 (***)

-0.82, -0.29

-0.11 (n.s.) -0.43, -0.01

-0.06 (n.s.) -0.31, 0.06

-0.16 (*) -0.54, -0.11

T 0.10 (*) -0.06 (n.s.)

-1.60, 0.78

0.17 (*) 0.11, 0.60

0.02 (n.s.) -0.19, 0.26

0.16 (*) 0.05, 0.57

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U 0.07 (n.s.) 0.04 (n.s.) -0.82, 0.78

0.17 (*) -1.14, 1.24

-0.02 (n.s.) -1.05, 0.71

-0.01 (n.s.) -1.61, 0.87 W1 -0.18 (***) -0.22 (**)

-0.66, -0.23

-0.05 (n.s.) -0.31, 0.12

-0.13 (n.s.) -0.44, -0.07

-0.30 (***) -0.81, -0.37 W2 -0.26 (***) -0.14 (n.s.)

-0.58, -0.00

-0.27 (***) -0.82, -0.24

-0.36 (***) -0.98, -0.45

-0.25 (***) -0.72, -0.26 WS -0.11 (**) 0.02 (n.s.)

-0.17, 0.26

0.07 (n.s.) -0.07, 0.34

-0.30 (***) -0.85, -0.35

-0.17 (*) -0.56, -0.14 X1 -0.12 (**) -0.13 (n.s.)

-0.42, -0.07

0.02 (n.s.) -0.07, 0.16

-0.14 (*) -0.51, -0.05

-0.21 (**) -0.66, -0.18

X2 0.39 (***) 0.33 (***)

0.40, 0.91

0.33 (***) 0.36, 0.94

0.31 (***) 0.38, 0.86

0.55 (***) 0.79, 1.41

X3 0.22 (***) 0.29 (***)

0.32, 0.85

0.35 (***) 0.43, 0.99

0.08 (n.s.) -0.07, 0.37

0.16 (*) 0.11, 0.52

Y 0.11 (**) 0.03 (n.s.)

-0.11, 0.23

0.23 (***) 0.22, 0.71

0.01 (n.s.) -0.15, 0.19

0.17 (*) 0.17, 0.52

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Supplement 3D Seasonal Mann-Kendall and Mann-Kendall trend analysis of functional evenness (FEVE), functional divergence (FDIV) and functional dispersion (FDIS) based on phytoplankton functional traits and phytoplankton functional groups sensu Reynolds in the middle Danube section, Göd (N-Budapest, Hungary) between 1979 and 2012. Tau and significance levels (in brackets) are given, as well as bootstrap confidence interval calculations (BCICs) for the Mann-K tests based on 10,000 bootstrap replicates at 99% CI (in second line). Significant trends are bold and italic.

Functional metric

GLOBAL (Seasonal M-K)

WINTER (Mann-K)

SPRING (Mann-K)

SUMMER (Mann-K)

AUTUMN (Mann-K) Functional traits

FEVE 0.21 (***) 0.00 (n.s.) -0.24, 0.24

0.03 (n.s.) -0.12, 0.24

0.36 (***) 0.48, 0.96

0.32 (***) 0.40, 0.89 FDIV 0.09 (*) 0.08 (n.s.)

-0.02, 0.36

0.14 (*) 0.11, 0.44

0.08 (n.s.) -0.04, 0.35

0.00 (n.s.) -0.13, 0.15 FDIS 0.31 (***) 0.14 (*)

0.09, 0.48

0.34 (***) 0.43, 0.92

0.21 (**) 0.20, 0.64

0.39 (***) 0.53, 1.02 Functional groups

FEVE 0.01 (n.s.) -0.23 (**) -0.71, -0.21

-0.18 (**) -0.53, -0.20

0.21 (**) 0.17, 0.67

0.27 (***) 0.29, 0.79 FDIV 0.13 (***) 0.18 (*)

0.16, 0.56

0.12 (n.s.) 0.03, 0.46

-0.07 (n.s.) -0.36, 0.09

0.31 (***) 0.38, 0.85 FDIS 0.19 (***) 0.14 (n.s.)

0.07, 0.47

0.32 (***) 0.38, 0.89

0.05 (n.s.) -0.13, 0.31

0.20 (**) 0.17, 0.62

References

Borics G., Várbiró G., Grigorszky I., Krasznai E., Szabó S. & Kiss Keve T. (2007) A new evaluation technique of potamo-plankton for the assessment of the ecological status of rivers. Archiv für Hydrobiologie, Supplementband Large rivers, 17, 466 - 486.

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Padisák J., Crossetti L. & Naselli-Flores L. (2009) Use and misuse in the application of the phytoplankton functional classification: a critical review with updates.

Hydrobiologia, 621, 1-19.

Reynolds C.S., Huszar V., Kruk C., Naselli-Flores L. & Melo S. (2002) Towards a functional classification of the freshwater phytoplankton. Journal of Plankton Research, 24, 417-428.

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