Salivary oral squamous cell carcinoma biomarkers – Exploring potential indicators in type-2 Diabetes
{ Jancsik V. A
1, Márk L
2, Molnár G.A.
3, Wittmann I
3, Olasz L
1}
Our study requires further validation with a larger population,but for the first time we detected overexpressed peptides and proteins from human saliva, which are possible predisponating biomarkers.
- The average age: 47,2 years (between: 23 – 76)
- Female – Male ratio: 45 - 55%
- Diagnosed with Diabetes ~ 18 years ( 0,5 – 26 years)
- No cancer registered in the past medical history
- Negative stomatooncological status, no smoker volunteers
D001 1,2,3 E001 negative
D002 1,2,3 E002 negative
D003 1,2 E003 negative
D004 1,2,3 E004 negative
D005 1,3 E005 negative
D006 1,2,3 E006 negative
D007 1,2,3 E007 negative
D008 1
2047.9
1213.7 1836.4 1407.8
2769.4
1082.6
2472.3 3039.6
0 1000 2000 3000 4000 5000
Intens.[a.u.]
500 1000 1500 2000 2500 3000 3500
m/z
H2 D3
Annexin - A11: Ca2+ dependent protein,
regulation of inflammatory pathways, detected in previously also in OSCC patients1
Peroxiredoxin-2: eliminates oxidizing agents caused by ionization, detected also in OSCC patients2
Tyrosine - protein phosphatase: found also in colon carcinoma, needs further analysis, to understand its role in the pathogenesis of OSCC3
1. Patients data
2. SDS-PAGE Electrophoresis
3. Autoflex II TOF/TOF – Bruker Daltonics®
1. The 7 spots of interest 2. The MS diagrams of the detected proteins
3. After searching them against the Mascot search engine:
3. Protein
2. Protein 1. Protein
I ntroduction
Recent conducted
epidemiological studies and animal experiments showed that there is a relationship between diabetes and oral squamous cell carcinoma. Our goal to find salivary biomarkers, which could refer to the risk of possible oral malignancy.
M ethods
Under standardised circumstances we collected saliva samples of diabetic patients (n=17). As a control group we used saliva from healthy subjects (n=15). The samples were analysed using SDS- PAGE electrophoresis and MALDI-TOF/TOF mass spectrometry after tryptic digestion. The resulted proteines were identified by peptide mass finger printing. The peptide masses were searched by utilising the MASCOT Server 2.2 search engine (p<0.05).
R esults
The analysis demonstrated a different pattern of protein biomarker expression between diabetic and control subjects. A significant difference was measured in the expression of annexin-A11, tyrosine- protein phosphatase and peroxiredoxin-2, which were previously found also in oral cancer patients.
C onclusion
1. Patients datas
Nr. Diabetic Nr. Control
SROP-4.2.2/B-10/1-2010-0029 Supporting Scientific Training of Talented Youth at the University of Pécs
