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j our na l h o me p ag e:w w w . i n t l . e l s e v i e r h e a l t h . c o m / j o u r n a l s / d e m a
Comparative mechanical behavior of dentin enamel and dentin ceramic junctions assessed by speckle
interferometry (SI)
Michel Fages
a,∗, Pierre Slangen
b, Jacques Raynal
a, Stephane Corn
b, Kinga Turzo
c, Jacques Margerit
a, Frédéric J. Cuisinier
aaEA4203,DepartmentofBiologicSciences,UniversityofMontpellierI,Montpellier,France
bEcoledesMinesd’Alès,I.L.O.A.,Alès,France
cDepartmentofProsthodonticandOralBiology,UniversityofSzegedDentalSchool,Szeged,Hungary
a r t i c l e i n f o
Articlehistory:
Received23October2011
Receivedinrevisedform3May2012 Accepted18May2012
Availableonlinexxx
Keywords:
Dentinenameljunction(DEJ) Dentinceramicjunction(DCJ) Speckleinterferometry(SI) Toothcrown
Ceramiccrown
a bs t r a c t
Objective.Thedentin–enameljunction(DEJ)playsacrucialroleindentalbiomechanics;how- ever,littleisknownaboutitsstructureandmechanicalbehavior.Nevertheless,naturalteeth areanecessarymodelforprostheticcrowns.ThemechanicalbehaviorofthenaturalDEJ andthedentinceramicjunction(DCJ)manufacturedwithaCAD-CAMsystemarecompared.
Methods.Thereferencesamplesundergonomodification,whiletheexperimentalsamples weredrilledtoreceiveacementedfeldspathicceramiccrown.Longitudinallycutsamples wereusedtoachieveaplanarobjectobservationandtolook“inside”thetooth.Acom- pleteapparatusenablingthestudyofthecompressivemechanicalbehavioroftheinvolved toothbyanon-contactlaserspeckleinterferometry(SI)wasdevelopedtoallownanometric displacementstobetrackedduringthecompressiontest.
Results.ItisobservedthattheDEJactedasacriticalzoneaccommodatingthemovement betweendentinandenamel.Asmoothtransitionoccursbetweendentinandenamel.In themodeledprosthetic,thesamekindofaccommodationeffectsalsooccurs,butwitha steepertransitionslopebetweendentinandceramic.
Significance. On thenatural tooth, thestress accommodation arises froma differential behaviorbetweenenamelanddentinfromtheDEJ.Intheceramiccrown,thecemented dentin–ceramicjunctionshouldplaythisrole.Thisstudydemonstratesthepossiblereal- izationofprostheticcrownreconstructionsapproachingbiomechanicalbehaviors.
©2012AcademyofDentalMaterials.PublishedbyElsevierLtd.Allrightsreserved.
1. Introduction
Thedentin–enameljunction(DEJ)inteethisthezonebetween twodistinctcalcifiedtissueswithverydifferentbiomechan- icalproperties:enamel and dentin[1]. Enamelishard and brittleandenvelopsthesofterdentin.Theenamelanddentin worktogetherduringthemanyloadcyclesexperiencedbythe
∗ Correspondingauthorat:11AvenueCelestinArnaud,34110LaPeyrade,France.Tel.:+33684855715;fax:+33467486092.
E-mailaddress:mifages@wanadoo.fr(M.Fages).
toothoveritslifetime.Generally,interfacesbetweenmaterials withdissimilarelasticandmechanicalpropertiesrepresent
“weak links” ina structure; however, the DEJacts to suc- cessfullytransferappliedloads(e.g.,masticatoryorimpact) from the enamelto thedentinand inhibits enamelcracks frompropagatingintothedentinandcausingtoothfracture [2,3].
0109-5641/$–seefrontmatter©2012AcademyofDentalMaterials.PublishedbyElsevierLtd.Allrightsreserved.
http://dx.doi.org/10.1016/j.dental.2012.05.006
Pleasecitethisarticleinpressas:FagesM,etal.Comparativemechanicalbehaviorofdentinenamelanddentinceramicjunctionsassessedby attheDEJ,thusexplainingwhysofewcrackingeventscause
delaminationwhentheyimpingeontheDEJ.Zaslanskyetal.
[8,9] highlighted the importance ofthe DEJ as the binding interfacebetweenenamelanddentin.Theyhaveshownthat adjacenttotheDEJisa200–300mm-thickzoneofdentinofa muchlowerstiffness(compressionelasticmodulus)thanthe bulkofthedentininthetooth.
Restorations that are all ceramic require proper adhe- sive bonding on the dentin to achieve their required life expectancy. All-ceramic restorations are made with felds- pathicor zirconiaceramics.Thestrongestceramicshave a fracturetoughnessofatleast3.0MPam1/2[10],whichisrel- ativelyclosetotheenamelfracturetoughnessof1.3MPam1/2, inadirectionperpendiculartotheenamelrods[8].Neverthe- less,fracturesoftheceramicpartofall-ceramiccrownsare difficulttoprevent, andcrack growth isasignificant prob- lem[11].Thisphenomenoncanbeexplainedbytheabsence ofastressaccommodationzone.Thenaturalstressaccom- modationzoneof200–300m-thickdentinhasamuchlower stiffnessthanthebulkofthedentincore[8].
Bondingagentsmust beselectedvery carefullybecause theydeterminenotonlytheadhesionbutalsotheultimate strengthoffull-ceramiccrowns[12–14];therefore,itisimpor- tant to compare the mechanical behavior ofnatural teeth andoftheall-ceramiccrowncementedondentin.Insteadof
“cementjoint”,wewillusetheterm“dentin–ceramicjunction”
(DCJ).
We applied compressive forces representative of those occurringintheoralcavityonnaturalteethandall-ceramic crowns,andwedeterminetherelativemovementofenamel anddentin,orceramiccrownanddentin,respectively.
2. Materials and methods
2.1. NaturalteethIntactlowerfirstpremolarsfreeofcarieswerestoredinphys- iological serum after havingbeen extracted as part ofthe routineorthodontic treatmentofyounghealthy adolescent patients(aged<18).Fivesetsoftwosampleseach(onenat- uraltoothandoneprosthetictooth)wereamassed.Rightand leftpremolarsfromthesamepatientwereused.Onewaskept intact,andtheotherwaspreparedtoreceivetheprosthetic crown.
prostheticcrowns[15].Thewholecloningprocessispresented inFig.1.
The Vita MarkII® (Vita Zahnfabrik, Bad Säckingen, Switzerland) ceramic blocks of albite-enriched feldspathic ceramicwereused.Theirabrasioncoefficientisclosetothatof naturaldentalenamel.Aftermilling,theextradoswereglazed (Azkent®,VitaZahnfabrik,BadSäckingen,Switzerland).
Thecrownswerecementedontothepreparedteethusing RelyxUnicem®adhesivecement(3MESPEDentalDivision,St.
Paul,MN,USA)followingthestandardclinicalprotocolofillu- minationofeachsideofthecrown for4sat3000mW/cm2 withaSwissmasterLight®lamp(E.M.S.,Nyons,Switzerland).
2.3. Specimenpreparation
Afterextraction,theteethweredisinfectedandstoredinphys- iological serum with traces of chloroform.The teeth were longitudinally cutinthe vestibular–lingualorientation,and oneofthetworesultingpartswasremovedwithadiamond disc.Longitudinalcutshavebeenusedtoallowplanarobser- vation and toappreciatethe differentbehaviors insidethe toothofthenaturalDEJandoftheDCJinterfaces.Thetooth wasthengluedintothesampleholderwithalayerofAraldite® (HunstmanAdvancedMaterials,TheWoodlands,Texas,USA).
Thesampleholdershavebeencastinchromiumcobaltusing the imprintofaroot. Themechanicalstabilityofthespec- imenholderswasvalidatedbyaspeckleinterferometry(SI) experiment.
2.4. Loadingsystem
The compression test device fulfills the high sensitivityof speckleinterferometryandcopeswiththerigidbodymotions ofthewholesystem.Thesampletoothcementedinthesam- pleholderwasplacedundertheforcetransducer(Model31, HoneywellInternational,Morriston,NJ,USA).Thismid-range precision miniature load cell isslowly translated vertically bythemotor(M-235®,PI.Karlsruhe,Germany).Thesystem cangenerateaforce-drivendisplacement(C-862MercuryPI, Karlsruhe, Germany) or simply a user’s displacement.The compression apparatus communicates with the computer throughaNIUSB-6251port(NationalInstruments,Austin,TX, USA)andisinterfacedwithanin-houseLabViewprogram.The entiremechanical systemwas boltedonto theholographic tabletop(Newport,Irvine,CA,USA).Verysmalldisplacement steps,assmallas1.6nm,cantheoreticallybeachieved.The
Fig.1–Cloningprocess:theintactnaturalcrownsample(A),opticalprint(B),shaping(C),opticalprintofthesecondtooth preparedtoreceivetheprostheticclone(D),adaptationoftheshapingonthetoothprepared(E),CADfinished,CAMready (F),ceramicblockinthemillingunit(G),theprostheticclonemilled(H),theprostheticclonecementedonthetoothprepared (I),thenaturaltooth(J),verticalcuts(K),prostheticclone(L)naturaltooth.
forcecanbeappliedtothetoothdirectlywiththeforcetrans- ducerorthrougharelayrod.Forcehasalwaysbeenapplied tothesamepartofthelingualcusp.PreliminarytestingbySI showedgoodperformancesofthemechanicalset-upandno spuriousdisplacements.
2.5. SIapparatus
Theopticalset-upwaspreviouslypresentedindetail[16].The frequency-doubledYAGlaseremits50mWat532nmwave- lengthinthegreenrange.Thelaserbeamistheninjectedin aCOTS(commerciallyoff-the-shelf)system(CanadianInstru- ments,Nottingham,UK)offeringinjection,variableintensity couplingintheoutputfibers,andphaseshifting.
Therearetwoinputfibers:oneforinjectionandonefor detectionofthereflectedsignalattheoutputfiberinterfaces.
Partsoftheoutputfibersarebaredandwrappedaroundpiezo- electrictransducers.Thephaseshiftisappliedoneitheror bothofthetwooutputfibersbyapplyingavoltageatthePZT andthusgeneratingatinyextensionofthefiber.Thesystem isprotectedfromthermalandmechanicaleffectsbyaplastic boxandiseasilybreadboardable.Phaseshiftswerecalibrated usingcommonprocedures[16].
Asensitiveopticalfiberin-planeinterferometerhasbeen designedwithsensitivityvectorSv.Horizontalsensitivityis achieved.Two symmetricalbeamsproducean interference signalonthewholeobjectandthenilluminatethesamples.
Theobjectsareopticallyrough-renderedbywhitepowder.An XC70CCDcamera(Sony,Tokyo,Japan)recordsthesamplesur- faceunderloadingforces.Theimagesarethenstoredinlive memoryoronthecomputer’sharddisk.
Theimageprocessingwasperformedusingtheappropri- ate software in LabView, and the results are presented as vectormapsorfalsecolormaps.Isodisplacementmapscan bevisualizedinrealtimeduringtheloadingofthesample.
Thesystemcanhandleareasrangingfrom5mm×5mmto 1m×1mwhenusingtheappropriatetypeoflaserandload- ingsystem.Themeasurementuncertaintyisapproximately 50nm, which iscommon forinterferometricmeasurement inacontrolledenvironment.Thedisplacementresolutionis
approximately10nm,whilethespatialresolutionisdirectly linkedtothemagnificationoftheobjectsceneontheimage sensor(1280×900pixels).
A “4-buckets” phase shifting algorithm leads to phase variationsduringthecompressiontest[17].Duringthetest, the initialphase statewas memory-resident and real-time subtractedfromthe currentstate.Sometimesthereference statewasalsorefreshedbecauseforsomeloadingsteps,the number offringescan betoohigh,and theresultingnoise wouldinterferewiththeinterpretationoftheresultingfringes.
Interferogramsshowthein-planedisplacementsfromphase shiftingspeckleinterferometry.Highestqualityimageswere storedontheharddiskandoverlaidwithupperjawposition andloadvalue(N).
2.6. Typicalexperiments
Specimens were white powdered using Eutest 3Developer Castolin Eutecticpowder (Castolin, Lausanne, Switzerland) togenerateauniformdiffusingsurfaceand toavoiddiffer- entmodulationsbetweendentinandenamelorceramicand dentin.Thesameloadswereappliedtothenaturaltoothand theprostheticcrownsamplestoallowcomparisonsoftheir respectivecompressivebehaviors.
Thestartingloadwasapproximately0N.Thecompression was increasedstepwise,performing discretedisplacements of the transducertip (one stepis approximately 1.6nmof Y-displacement).Therefore,agreaternumberofstepscorre- spondstoahighercompression.Thesamplecanbeloaded orunloaded.Theloadingrangeappliedtothedifferentsam- pleswasbetween5Nand120N.TheCCDcamerarecordsat thesamplesurfacetheinterferencesofthetwoillumination beamscomingfromthetwooutputopticalfibers.Livefringes aredisplayedbetweenareferencestateandthecurrentload state.Betweenminimalandmaximalloading,differentphase mapsarerecordedandstoredinthememory.
Themechanicaldeformationsarecomputedfromthedis- placementmapsgeneratedfromthephasedifferencemaps.
Speckle interferometry is a relative displacement mea- surement. The maximum range between two successive
Pleasecitethisarticleinpressas:FagesM,etal.Comparativemechanicalbehaviorofdentinenamelanddentinceramicjunctionsassessedby measurementsisapproximately20m.Inourexperiments,
weneedtorecord smallerstepsassomemechanicalnoise appears.Therefore, newdisplacement references(zerodis- placementreset)arerecordedduringthetest.
2.7. Displacementcalculations
Different operation modes of SI are commonly used, e.g., subtraction-mode, time-averaged SI, and double-pulsed SI [16].Inthiswork,wefocusonsubtraction-modeSI,ormore specifically, onphase-shifting SI, which is mainlyused for staticdeformationmeasurements.
Combining the primary interference pattern phase changes between the recordings yields new secondary interferencefringes(alsocalledcorrelationfringes).
Thevariableϕsdenotesthestartphase(alsocalledspeckle phase)attheinitialstateoftheobject.Thevariableϕrepre- sentsthephasechangebetweentwostates.
Thesespeckleinterferogramscanbesubtractedandlead tothefollowingequationforthesecondaryinterferencefringe pattern,assumingperfectspatialcorrelationbetweenthetwo primaryspecklepatterns:
I1−I2=2
IrIo(cos(ϕs+ϕ)−cos(ϕs))
Currently,noiselimitstheaccuracyofintensitysubtraction SItoapproximately15nm.Regardingthein-planesensitivity ofthesetup,theangleoftheimpingingbeamisapproxi- mately30◦anddeterminesthecorrespondencebetweenthe phasegraylevelvariationandthein-planedisplacementux.
Inourinterferometer,thewavelengthofthelaseris532nm, anditsrelationshipwiththein-planedisplacementisgivenby thefollowingequation:
ux= 4sinϕx
The resulting variationof onegray level ofphase ux is approximately2.08nm.
3. Results
Five setsoftwosampleseach(naturaltoothand prosthetic crown)wereproduced.Theexperimentalprotocolwastested andvalidatedwithfourofthem.Presentedresultscorrespond solely tothe fifthsample(for natural tooth and prosthetic clone).
Theinterferometricimagesdistinctlyshow thebehavior of the samplesand confirm the quality ofthe mechanical apparatusandthe integrityofthededicatedsampleholder (Figs.2and3).
AnaturaltoothispresentedinFig.2.Figurecaptionsalso presentthescreenshotnumber(orimagenumber),theforce appliedtothesample,theforcedifferencefromthereference state,andforcefromthereferencestate(orphasereset).
Fig.2Adisplaysthesampleunderwhitelightbeforepaint- ing.Fig.2B–DisSIimagesrecordedatdifferentcompression levels and present typical fringes. Fringesoccur when the displacement inducesan optical phaseof 360–0◦. The dis- placement inthe Xdirectioniscomputed from theoptical phase.
InFig.2B–D,differentcontinuousgraylinesdemarcatethe DEJ(redarrows)correspondingtotheimagetakeninwhite
Fig.3–Prostheticcrownsampleunderdifferentloads.(A)Whitelightimages.(B)Screenshotno.08:force36.78N,F:
2.57N(newref:41.01N).(C)Screenshotno.15:force77.56N,F:5.29N(newref:82.85N).(D)Screenshotno.33:force 64.09N,F:18.76N(newref:41.01N).
Fig.4–Naturaltoothbehaviorataloadof38.65N,F=1.74N,newref:40.39N.(A)SIimage:thesinglewhiteline
correspondstotheregionofinterest(ROI)usedforthecalculationofdisplacement.(B)Displacementcurvealongthewhite line,displacementsinnmversuspositioninpixels.Stepisabout43nm.
light(2A).TheDEJisclearlyvisiblewhentheappliedforce reaches35.5N.TheDEJisalwaysmorevisibleinfrontofthe loadingpoint.
Fig.3Apresentsaprosthetic crownsampleunderwhite lightbeforepainting,andFig.3B–Darerecordedatdifferent compression levels and present typical fringesatdifferent loads.
TheDCJappearsfrom38NandisclearlyvisibleinFig.3B–D (redarrows).Fortheprostheticcrowns,itappearsasacontin- uousgraylinecorrespondingtotheimagetakeninwhitelight (Fig.3A).
TheSIimagesdisplayedinFig. 2B–Dshowthatthenat- uralenamelcapmovesindependentlyfromthedentin.This differenceisclearlydelimitedbyalinecorrespondingtothe anatomicallocationofthedentin–enameljunction(DEJ).For theprostheticcrown,intheSIimages(Fig.3B–D)thesame kind ofshift occursatthe cement junctionofthe ceramic crownwiththedentin.TheDEJislesswellmarkedthanthe DCJduetothesmallerassociateddisplacements.Largevaria- tionsinintensity(Fig.3CandD)correspondtofringesresulting fromrigidbodymotion(inplanerotationprojectedontothe sensitivityvector).
Thehighestloadingvaluesenablingthedistinctionofthe interfacezonewere117.4Nfortheprostheticcrownand82.5N forthenaturaltooth.Beyond120N,allsamplesbehavedlike rigidbodies.Around200N,somesamplesweredestroyeddue tofracturesofthe brittlematerials.From allofthescreen- shots,differentimageswerechosenforuseincomputingthe displacementmaps.
In Fig. 4A, from left to right, we denote the transition betweenlightgrayanddarkgraycorrespondingtotheregion betweenthedentinandtheenamel.Onehorizontalwhiteline hasbeen definedasthe regionofinterest(ROI).In Fig.4B, the curve representsthe displacement change innanome- tersversusthepositionalongthewhitestraightlineshown inFig.4A.Therelativedisplacementbetweenthedentinand
the enamel is 52nm for loads between 38.6N and 40.4N (F=1.8N).
InFig.4B,thegraylevelarerisingattheendofthecurveas itisclosetotherightedgeofthetooth,itisclearlyvisibleon thenativeimagewithhighermagnification.Thisisgenerated bythesmoothinplanetiltofthepalatinecuspidfollowingthe upperdisplacementoftheupperjawrodfromthemechanical testingastheforcedecreasesfrom40.39Nto38.65N.
InFig.5A,infrontofthecuspsubjectedtotheload,the delineationmadebytheDEJisvisible,andthegraylevelsdiffer becauseofadifferentaccommodation.
Tocalculatethedisplacementvalue,sixredequalparal- lellinesweredefinedperpendicularlyacrosstheDEJ.Thered linesareseparatedby1pixelonetotheother,andsoappear asaboldredlineonthefigures.Thedisplacementistheaver- agevalueofthesix-stackedprofiles.InFig.5B,theblackcurve isthemeanvaluefittingofthebluecurvevaluesalongthe sixpaths,andthebluecurveisoneofthesixdisplacement curves.Themeanrelativedisplacementbetweenthedentin andtheenamelisabout20nmforloadsbetween39.21Nand 40.39N(F=1.18N).
Thesameanalyticalprocesswasappliedfortheprosthetic crowns(Figs.6–8).
In Fig.6A, onehorizontalblack linewas definedas the regionofinterest,asthetechnicalnoisewaslessprominent than forthe natural tooth.The area of interest is located inafringeinfrontofthestresszonefromleft toright.We denotethetransitionbetweenlightgrayanddarkgraycorre- spondingtotheregionbetweenthedentinandtheceramic cap.InFig.6B,thecurverepresentsthedisplacementchange innanometersversusthepositionalong thewhite straight lineshowninFig.6A.Therelativedisplacementbetweenthe dentinandtheceramiccapis43nmforloadsbetween82.8N and76.3N(F=6.5N).
InFig.7,toevaluatethedisplacementvalues,sixequalpar- allelstraightpathshavebeendefinedintheregionofinterest
Pleasecitethisarticleinpressas:FagesM,etal.Comparativemechanicalbehaviorofdentinenamelanddentinceramicjunctionsassessedby Fig.5–Naturaltoothbehaviorataloadof39.21N,F=1.18N,newref:40.39N.(A)SIimage:sixredequalparallellines, 1pixelseparated,aredefinedacrosstheDEJ.(B)Displacementcurves:bluecurve:displacementvaluesalongoneofthesix redlines.Blackboldcurveisthemeanvaluefittingofthe6bluecurves.(Forinterpretationofthereferencestocolorinthis figurelegend,thereaderisreferredtothewebversionofthearticle.)
acrosstheDCJinthepalatinezoneinfrontoftheloadingpoint (Fig.7A).ThesixredlinesaredrawnperpendicularlytotheDCJ toaccuratelymeasurethedisplacementvariationacrossthe DCJ.Thisismuchappropriatethanthesinglehorizontalblack lineforFig.6astheredlinesenabletherealcomparisonfrom onesidetotheotherperpendiculartotheDCJ.
InFig. 7B,the displacement isdisplayedas the average valueofthesixstackedprofiles.Theblackcurveisthemedian valuefittingofthebluecurvevalues.Inthiscase,therelative displacementisapproximately95nmforloadsbetween82.8N and72.8N(F=10N).ItshowsclearlytheeffectoftheDCJact- ingasanaccommodationareafortheapplieddisplacement, butwithasharperslopethanthenaturalDEJ.
TheSIimageinFig.8wasselectedtorepresentthecharac- teristicisodisplacementmapofthediscontinuityzonelocated infrontoftheloadingpointforaceramiccrown.Acolored andzoomedimageisalsopresented.Thefringeshiftinthe
region ofinterest clearlydelineates amechanical interface betweentheceramicandthedentin,whichareseparatedby thecement.
Fig.8presentsthecharacteristicfringesobtainedforahuge load difference(30.07N) andthe importantnoise occurring duetosomespeckledecorrelationeffect.ResultsfromFig.8 showitismandatorytomakestepreferencesenablingsmaller displacementloadandthusverylowdecorrelationnoise.
Fig.9showsthenaturaltoothwiththesamechargethan prosthetictooth(Fig.8)andallowstocompareDEJandDCJ.
4. Discussion
TheimportanceoftheDEJasaninterfacebindingtheenamel and dentinsurfaceshaslong beenrecognized(Tylman [2]).
OurunderstandingoftheroleandthelocationoftheDEJhas
Fig.6–Prostheticcrownbehaviorataloadof76.34N,F=6.51N,newref:82.85N.(A)SIimage:asingleblackline correspondstotheregionofinterest(ROI)usedfordisplacementcalculation.(B)Displacementcurvealongtheblackline, displacementsinnmversuspositioninpixels.Stepisabout43nm.
Fig.7–Prostheticcrownbehaviorunderaloadof72.85N,F=10N,newref:82.85N.(A)SIimage:sixredequalparallel lines,1pixelseparated,aredefinedacrosstheDEJ.(B)Displacementcurves:bluecurve:displacementvaluesalongoneof thesixredlines.Blackboldcurveisthemeanvaluefittingofthe6bluecurves.Thedisplacementisapproximately95nm.
(Forinterpretationofthereferencestocolorinthisfigurelegend,thereaderisreferredtothewebversionofthearticle.)
evolvedwithongoingresearch.Theuniquemechanicalprop- ertiesofthiszonewerefirstrecognizedfrommicro-hardness profiles(Craigetal.[18,19]).WangandWeiner[7]measuredthe strainacrossthiszonewhenacompressiveloadwasapplied andsuggestedthatthisisanimportantworkingpartofthe toothduringmastication.Anasymmetrybetweenenameland dentinwasnotedpreviously(Wood etal. [20])but was not quantified.Bechtleetal.explainedthephenomenonofcrack arrestattheDEJusingtheelasticmodulusmismatchbetween thedentinandtheenamel[21].
It was found (Zaslansky [9]) using SI that a com- pressive load applied to the tip of the main cusp of a human premolar caused the entire enamel cap to
move essentially as a stiff body, tilting toward the buccal surface.
Recently,Barak etal. [22]highlighted theimportanceof enamelinawhole-toothdemonstrationthroughafiniteele- mentmodelstudyandvalidatedtheirfindingsbyametrology method.Chattahetal.[23]showedthattheenamelcapina minipig animalmodeliscapableofdeformingandrotating atloadsaslowas16N.Zaslanskyetal.[9]showedthatthe enamelcapofanisolatedhumanpremolardidnotdeformor rotateatloadslowerthan80N.Humanenamelisstifferthan thatoftheminipig,andthecuspsdonotdeformorcrackuntil highloadsarereached.However,inbothcases,theaimwasto preservethefunctionalityofthegrindingsurfacesoverlong
Fig.8–SIimagesample“prostheticcrown”anddisplacementsshowninacontourcoloredmap.Behaviorforaloadof 52.78N,F=30.07N,newref:82.85N.
Pleasecitethisarticleinpressas:FagesM,etal.Comparativemechanicalbehaviorofdentinenamelanddentinceramicjunctionsassessedby Fig.9–SIimagesample“naturaltooth”.Behaviorfora
loadof52.3N,F=6.5N,newref:58.8N.
periodsoftime.Theuseoftheseopposingstrategiestoachieve thesameendhasbeenattributedtophylogeneticdifferences inmasticatoryfunction.[24,25].Theminipigmolariscapable ofdeformingandrotatingatlowloads,andtheintrinsicreac- tionofthecrowntoeccentricloadingiscomplementedand evenenhancedbythestructuressupportingthetooth[23].
Inourstudy,weconsideredthehalf-cutcrownbecausethis configurationhasthemajoradvantageofobserving thein- planebehavioroftheDEJandtheDCJinanddoingsofrom eitherfarfromorclosetotheloadingzone.Zaslanskyworked withparallelepipedcutsfrom premolars[9], whileChattah etal.[23–25]studiedwholeteeth.OnSIimages,theinterfaces appeardistinctlyalongtheirentirelengths,thusshowingtheir completeloading(from35.5NfortheDEJonthenaturaltooth andfrom 36Nfortheprosthetic crown).Thefirstinterpre- tationconfirms thatthe enamel bulkand the ceramiccap willmoveslightlyunderloadingasrigidbodies.However,the mechanicalresponseisdifferentforthesameappliedload- ingforce,andthedisplacementislargerforceramicthanfor enamel.ThusinFig.8,ataloadof52.7N,theSIimageclearly showsarelativedisplacementbetweenenamelanddentin.In Fig.9,atasimilarload(52.3N),theimageidentifiestheDEJand thedifferingbehaviorsoftheenamelanddentin.However,it doesnotshowarelativedisplacementashighasinFig.8.This displacementisconfirmedinSIpicturesinFigs.6and7,with valuesof83nmand95nm,respectively.Thisbehaviordemon- stratestheaccommodationstrengthoftheDCJandconfirms itsprotectiverolefortheceramiccaps.
Moreover, we emphasize that the displacement of the crown,whetherenamelorceramic,isdifferentifthemea- surementareaisfarfromorclosetotheloadingzone.Ineach case,thelargerdisplacementislocatedoppositetotheload.
Thegraylevelsmapsalsoshowadisplacementoftheopposite cuspidbutofsmallermagnitude.
For the natural tooth, Zaslansky writes, “the asymme- try in stiffness between the buccal and lingual sides may
tainload(117.4Nfortheprostheticcrownand82.6Nforthe naturaltooth),theSIimagesshowthattheDEJandtheDCJ cannotbeobserved.Whentheinterfacesinbothsettingscan nolongeraccommodatetheloadingstress,theteethbeginto actaswholerigidobjects,andhigherloadingforcesgenerate cracksandfracturesofthesamples.
OurworktendstoconfirmbothZaslansky’sandWeiner’s studies:theDEJzoneisanimportantpartofthetoothstruc- ture.Moreover,wehavedemonstratedacomparablebehavior fortheDCJ.Webelievethesezonesarecrucialforthestress resistanceofthecrownstructure,whether naturalor pros- thetic.Formonoblocvitreousceramicprostheticcrowns,load resistanceisaresultofthecementingprocess[27,28].
ThesoftDEJinterfaceisactuallyagradedstructure,and muchremainstobeunderstoodaboutthemannerinwhich thewholetoothbehavesunderload.Figs.6and7showthat theDCJpresentsagradedbehaviorbutwithasharperslope thantheDEJ.Aninterestinguseofdento-prostheticspacing couldbetoselectthecementjointthicknessbaseduponthe cementelasticmodulus.Thiswouldrefineattemptstomimic thephysiologicalbehaviorofthenaturaltooth[29],accord- ingtothebiomimeticconcept[30].Thisseemsalreadyvery importantasseveralauthorshaveinvestigatedtheeffectof differences in the resin-cement elastic moduluson stress- transmissiontoseveralcompositeorceramicreconstructions [31,32]. Other authors have studiedthe influence ofdiffer- entbondingagentsunderstressontheinternalandmarginal adaptationofcompositeorceramicreconstructions[33].
Thebiomimeticprinciplethroughtheuseofreconstruction ceramicsthathavewearcoefficientsclosetothatofnatural enamelresultingfromcementswithanelasticmodulussim- ilartothatofthenaturalDEJ,wecanexpecttheconstruction ofrealbiomimeticprostheticteethinthenearfuture.
5. Conclusion
Speckleinterferometryishighlyrecommendedforperforming displacement measurements fordental biomechanics.The SIapproachallowsforthemeasurementofthemechanical propertiesofbiologicalstructuresandotherbiomaterialsthat areafewhundredmicronsthick.Thestrainaccommodation capacityofthetoothisderivedfromthedifferentialdisplace- mentbetweentheenamelandthedentin.Thedentin–enamel junctionactsasaninterfaceuntilacertainmaximalloading.
Beyondthisthreshold,theloadingaccommodationproperty
disappears,andthetooththenbehavesasarigidbody.We demonstrateasimilarbehaviorfortheprostheticcrowntooth.
Inthiscase,theenamelisreplacedbytheceramiccrownand thedentin–enameljunctionbythedentin–cementjunction.
ThesebehaviorsindicatetheinterfaceroleoftheDEJandthe DCJinthecapacityofteethtoaccommodatethestressesof theirphysiologicalfunctionsorevenoftheirparafunctions.
Forthecementcharacteristicsandthecementthicknessused inthis paper, the accommodation effect ofthe DCJ isless markedthanthatoftheDEJ.Futurestudywilldealwiththe effectofthecementcharacteristicsandthecementthickness byapplyingSIforsampleshavingdifferentcementsanddiffer- entthicknessofcementjoint,thankstotheCAD/CAMsystem.
ThelimitsofSImeasurementarethesensitivitytorigidbody motionanddecorrelationnoiseaffectingthespatialresolu- tion.Thiscanbeenhancedbyusingsmallerpixelsensorswith higherphotographicmagnification.
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