TÁMOP-4.2.2/B-10/1-2010-0012 project: “Broadening the knowledge base and supporting the long term professional sustainability of the Research University Center of Excellence at the University of Szeged by ensuring the rising generation of excellent scientists.”
Expression and functional studies on the PRINS non-coding RNS
Anikó Göblös1, Sarolta Bacsa1, Zsuzsanna Bata-Csörgő1,2, Lajos Kemény1,2, Márta Széll2
1Department of Dermatology and Allergology, University of Szeged, Szeged, HUNGARY
2Dermatological Research Group of the Hungarian Academy of Science, University of Szeged, Szeged, HUNGARY
Our research group has previously identified and characterized a non-coding (nc) RNA molecule, called PRINS (Psoriasis Susceptibility-related Non-coding RNA). Our in vitro experiments showed that PRINS ex- pression is induced by various stress factors. Next we demonstrated that nucleophosmin (NPM) is a direct interacting partner of PRINS. It has been previously demonstrated that the nucleolar–nucleoplasmic shuttling of the multifunctional phosphoprotein NPM is affected by ultraviolet (UV) light in fibroblasts and cancer cells.
We aimed to examine the cellular and histological distribution of PRINS ncRNA using in situ hybridization and to study the role of PRINS in the intracellular trafficking of NPM.
The in situ hybridization experiments demonstrated variable staining pattern in human tissue samples:
high levels of PRINS were detected in the gut, lungs, lymph nodes, uterus, testicles and skin, however no staining was observed in the cerebrum and cerebellum. The epidermis and the in vitro cultured keratino- cyte cells exhibited strong nucleolar and perinuclear expression and a moderate homogenous cytoplasmic staining.
Gene specific silencing of the PRINS expression in UV-B-irradiated keratinocytes resulted in the retention of NPM in the nucleolus, suggesting that PRINS is physically and functionally linked to NPM.
Taken together, in situ hybridization experiments demonstrated that PRINS is expressed in various tissue types but the level of expression shows great variety. Functional experiments suggest that PRINS may play a role in NPM-mediated cellular stress response in the skin.
