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Szeged - Timisoara axis for the safe food and feed

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Szeged - Timisoara axis for the safe food and feed

Acronym: SZETISA 1

HURO / 0901/147/2.2.2

Szeged University

Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara

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Determination of microelements as affected by microbial toxins in wheat

2 wheat cultivars artificially infected with 2 Fusarium strains of low and high activities, respectively, in Szeged (Cereal Research Institute).

Wheat specimen spontaneously and naturally infected - collected in Romania The analyzed material

Artificially infected wheat cultivars – moment 0 – Fusarium strain identification the samples were negatives

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GMO presence

Fusarium detection

Toxin genes identification Corn + Soy bean

Fusarium graminearum Fusarium culmorum Fusarium proliferatum Fusarium verticiloides

TRI 7 DON TRI 13 DON TRI 7 NIV

PQF5 - fumonisin

8 samples of commercial feed samples

Wheat specimen spontaneously and naturally infected - collected in Romania

Fusarium detection

Toxin genes identification Collected from the last year’s harvest

7 wheat samples, collected from three Romanian counties

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WHEAT SAMPLES

18 wheat samples, collected from two Romanian counties

Fusarium detection

Toxin genes identification Toxin quantification

Flag leaf Culm

Ear Chaf

Grain Fusarium quantification

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The analyzed material

DETERMINATION OF MICROELEMENTS AS AFFECTED BY MICROBIAL TOXINS IN MAIZE.

2 corn cultivars - genetically modified (Bt) and conventional from different locations

5 maize samples from last year’s harvest 4 conventional varieties

YG – genetically modified maize

GMO presence

Fusarium detection

Toxin genes identification

Maize samples from this year harvest conventional varieties

genetically modified maize

GMO presence

Fusarium detection

Toxin genes identification Toxin quantification

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SIGMA

SATELIT

Two month treatment

The plants are in the green house ALFALFA metal treatment

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INCREASE OF INFRASTRUCTURE

COOLING CENTRIFUGE - SIGMA

Multi-Sample Micro-Volume UV-Vis Spectrophotometer

The tender was organized The contract was signed

The tender was organized The contract was signed

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•Measure nucleic acid and protein concentration of samples as small as 1 µL

•Direct, easy measurements from tubes or plates

•Single sample mode or up to eight samples at a time

•Analysis of 96 samples in less than 6 minutes

•Full spectral output

•Calculates sample purity ratios (260/280)

•Pre-configured methods for common applications such as Nucleic Acid, Protein A280, Microarray, Proteins & Labels, Bradford, BCA, Lowry and Pierce 660nm – and more

•Intuitive and flexible software for creating custom methods and exporting data

•No consumables

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THANK YOU FOR YOUR

ATTENTIONS!

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