AERATION
-AGITATION
The role of oxygen
The role of oxygen , , respiration, oxygen demand respiration, oxygen demand
Aeration agitation Aeration agitation
B
Bioreaktor ioreaktorss
MASS TRANSFER UNIT OPERATIONS
MASS TRANSFER UNIT OPERATIONS Scale upScale up
Role of oxygen
Role of oxygen , , respiration respiration
RESPIRATION is an energy yielding metabolic process , in which an organic or inorganic compound (energy source) is oxidized by the organism with an inorganic compound.
If oxidizing agent is not oxygen, it is an If oxidizing agent is not oxygen, it is an
ANAEROB
ANAEROBICIC RESPIRRESPIRATIONATION If it is oxygen, we call it
AEROB
AEROBICIC RESPIRRESPIRATIONATION
Energy source Oxidant Products of example (reducing=oxi- (terminal elect- respiration
dized compound) ron acceptor)
H2 O2 H2O Hydrogen bacteria
*H
*H22 SOSO4422-- HH22O+SO+S22-- DesulfovibrioDesulfovibrio
NH3 O2 NO2- + H2O Nitrifiing bacteria
NO2- O2 NO3-+H2O Nitrifiing bacteria
Role of oxygen, respiration Role of oxygen, respiration
NO O2 NO +H2O Nitrifiing bacteria
*
*organic cpd.organic cpd. NONO33-- NN22+CO+CO22 DenitrifiDenitrifiinging babacteriacteria
Fe2+ O2 Fe3+ Ferrobacillus
S2- O2 SO2 + H2O Thiobacillus
organic compound O2 CO2+H2O most of microorg.,plants and animals
Role of oxygen, respiration
Role of oxygen, respiration
Role of oxygen, respiration Role of oxygen, respiration
glükóz (6 C-atom)
G-6-P F-6-P
F-1,6-diP
Gliceraldehid-P (3C-atom)
ADP ATP
ADP ATP
ATP ADP
2-P-glicerát 3-P-glicerát
1,3-diP-glicerát 2H2H
PEP Pyr Ac-CoA
citrát
2H2H
Oxálacetát
Cis-akonitát
i-citrát
αααα−−−−keto-glutarát
Szukcinát Fumarát
Malát
ATP ADP
NAD
koenzimQ 2*3 CO2 + 5*2H = C6H12O6
CO2 CO2
CO2
2H2H
2H2H 2H
2H
2H2H
0,4
KJ
0,27 V 51,2 kJ NAD
NAD FP1 KoenzimQ cyt b cyt c cyt a cyt a3
ATP ATP
ATP
FP2
2e-
1/2O2
2H+
H2O
Role of oxygen, respiration Role of oxygen, respiration
84 168
-0,8
51,2 kJ
0,22 V 41,6 kJ
0,53 V 100 kJ Q
b
c
a
a3 ADP + Pi ATP + H2O ∆Gο=+7,3 kcal =30,7 kJ
Role of oxygen, respiration Role of oxygen, respiration
OX
OXYGEN DEMANDS OF MICROORGANISMSYGEN DEMANDS OF MICROORGANISMS
Oxygen can be limiting substrate
Oxygen demand is expressed in two ways:
1. Respiration rate =
dc dt
[[[[ mmol O2/ dm3.h]]]],
[[[[kg O2/ m3 .h]]]]
dc 1
2. Specific respiration rate
Role of oxygen, respiration Role of oxygen, respiration
Q x
dc
= 1 dt
[[[[ h-1 ]]]]
dx dt
c
K c x
O
= µ
max+
2
Y x
O
= ∆ c
∆
dc dt
1 Y
dx dc
1 Y
c
K c x
O O
max
O2
= − = −
µ +
Q 1 x
dc dt
1 Y
c
K c
O
max
O2
= = −
µ +
Role of oxygen, respiration Role of oxygen, respiration
O O2
Q ≅ Q max
1 1
Y Y
m
O OG
= max + O
µ
First order Kinetics
Oth order Kinetics
Q
maxQ ≅
c K
Q c dt
dc x Q 1
O2
max
+
=
=
O2
max
K Q c
Q ≅
µµµµµµµµmax max -- SPECIFIC GROWTH RATESPECIFIC GROWTH RATE Y
Yoo -- OVERALL YIELD OVERALL YIELD COEFFICIENT COEFFICIENT FOR OXYGEN FOR OXYGEN m
mOO –– specific maintenance coefficient for oxygenspecific maintenance coefficient for oxygen
[[[[[[[[g0g022/g /g cellcell.h.h]]]]]]]]
--maximal yield for oxygenmaximal yield for oxygen
max
Y
OGRole of oxygen, respiration Role of oxygen, respiration
Q
Qmaxmax –– maximal specific oxmaximal specific oxyyggeen n demand or sp. resp. ratedemand or sp. resp. rate K
KO2O2 -- substrate saturation constantsubstrate saturation constant C
Cccrr –– critical oxygen concentrationcritical oxygen concentration
OG
Specific resp rate Specific resp rate
Glucose Oxygen Concentration in broth 1% ≈104 mg/dm3 7 mg/dm3
Critical concentration 50 mg/dm3 0,7 mg/dm3 Specific consumptio
rate 580 mg/g.h 208 mg/g.h
S U P P L Y S U P P L Y
rate 580 mg/g.h 208 mg/g.h
