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Figure 1

A B

C

Days after plating

3 5 7 9

C57Bl/6CD-1

A

Percentage of LGR5 positive cells (%)

CD-1 C57Bl/6

B

Percentage of CK14 positive cells (%)

C

Percentage of LGR5+CK14+ positive cells (%)

CD-1

Figure 3

A B C

M Slc9a1 Slc9a2 Slc26a6 CFTR Slc4a4 M Slc26a3 ANO1 O K O K O K O K O K O P O P

M Slc9a1 Slc9a2 Slc26a6 CFTR Slc4a4 M Slc26a3 ANO1

C57BL/6

CD-1

O K O K O K O K O K O P O P

Figure 4

B C

6.50 7.00 7.50 8.00 8.50

pHi

Na⁺-free

Hepes

NH₄Cl NH₄Cl NH₄Cl NH₄Cl 20 5 15 10 25 5 20 15 25 10

100 150 200 250 300 350 400

ering capacity (mM/pHU)

ß_HCO3 -ß_i

ß_total 3.0

4.0 5.0 6.0 7.0 8.0

440/495

3 min 3.0

4.0 5.0 6.0 7.0 8.0

440/495

Hepes

7.2 7.4 7.6 7.2 7.4 7.6

3 min

Hepes

7.2 7.4 7.6 7.2 7.4 7.6

A

Figure 5

Figure 6

Hepes

6.0 6.5 7.0 7.5 8.0 8.5

pH

i

6.0 6.5 7.0 7.5 8.0 8.5

pH

i

Hepes

Na⁺-free

NH₄Cl NH₄Cl Na⁺-free NH₄Cl Na⁺-free

1 µM HOE-642 50 µM HOE-642

Na⁺-free

NH₄Cl NH₄Cl Na⁺-free NH₄Cl Na⁺-free

1 µM HOE-642 50 µM HOE-642

3 min 3 min

B A

Hepes

100 150 200 250 300

J(B-)

C57BL/6 CD-1

Figure 7

B A

6.8 7.0 7.2 7.4 7.6 7.8 8.0 8.2

pH

i

6.6 6.8 7.0 7.2 7.4 7.6 7.8 8.0 8.2

pH

i

In document MOUSE ORGANOID CULTURE IS A SUITABLE MODEL TO STUDY (Pldal 28-41)