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Artificial hybridization of Japanese and European eel (Anguilla japonica×A. anguilla) by using cryopreserved sperm from freshwater reared males

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Müller Tamás (2012. 06. 20.)

Artificial hybridization of Japanese and European eel (Anguilla japonica×A. anguilla) by using cryopreserved sperm from freshwater reared males

Abstract

Eggs of Japanese eel (Anguilla anguilla) were fertilized with cryopreserved sperm of European eel (A.

anguilla) obtained from fish reared and matured in freshwater conditions.

A new genomic PCR-RFLP marker was developed to distinguish the two species and detect maternally and paternally derived DNA fragments to genetically verify the hybrid nature of the offspring.

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