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Manifestation of Novel Social Challenges of the European Union in the Teaching Material of Medical Biotechnology Master’s Programmes at the University of Pécs and at the University of Debrecen

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(1)

Medical Biotechnology Master’s Programmes

at the University of Pécs and at the University of Debrecen

Identification number: TÁMOP-4.1.2-08/1/A-2009-0011

(2)

CELL-SCAFFOLD INTERACTION

Dr. Judit Pongrácz

Three dimensional tissue cultures and tissue engineering – Lecture 11

Manifestation of Novel Social Challenges of the European Union in the Teaching Material of

Medical Biotechnology Master’s Programmes

at the University of Pécs and at the University of Debrecen

Identification number: TÁMOP-4.1.2-08/1/A-2009-0011

(3)

Scaffolds I

• Pre-made network of biomaterial

• One cell type seeded on one scaffold

• Several cell types seeded on a scaffold simultaneously or sequentially

(4)

TÁMOP-4.1.2-08/1/A-2009-0011

Scaffolds II

CO2

Fused beeds Fused irregular Fused cube porogen Cube porogen Fleece

(5)

Cell line cells nestling on a scaffold

A549

(lung adenocarcinoma cell line)

(6)

TÁMOP-4.1.2-08/1/A-2009-0011

Matrigel®

Matrigel:

• is the trade name for a gelatinous protein mixture secreted by mouse sarcoma cells

• This mixture resembles the complex extracellular environment found in many tissues

• Matrigel is marketed by BD Biosciences

(7)

Primary SAEC (Small Airway

Epithelial Cells) in matrigel

(8)

BIOFACTORS

Dr. Judit Pongrácz

Three dimensional tissue cultures and tissue engineering – Lecture 12

Manifestation of Novel Social Challenges of the European Union in the Teaching Material of

Medical Biotechnology Master’s Programmes

at the University of Pécs and at the University of Debrecen

Identification number: TÁMOP-4.1.2-08/1/A-2009-0011

(9)

Biofactors

PI3K

Wnt

GSK3

ATF2 b-TrCP

Wnt

TCF

PLCg

TAK SMAD Elk1

ATF2 c-jun c-fos

Hey

HES Frizzled

Disheveled

FGF

FGF receptor

BMP

TGF-b

BMP and TGF-b receptor

Notch

Jagged or Delta-like ligands

ICD Cytosol

Nucleus

Adjacent cell

b-catenin

accumulation b-catenin

degradation

Proliferation Differentiation

Migration

(10)

TÁMOP-4.1.2-08/1/A-2009-0011

Main growth factors (GFs) used in tissue engineering

VEGF – vascular endothelial growth factor KGF – keratinocyte growth factor

TGF-b – transfroming growth factor beta BMP – bone morphogenic protein

FGF – fibroblast growth factor

PDGF – platelet-derived growth factor

(11)

VEGF

• Primarily in endothelial cells

• Dimeric glycoprotein

• Four splice variants

• Receptors: VEGFR-1 and VEGFR-2

(12)

TÁMOP-4.1.2-08/1/A-2009-0011

TGF-b

• Superfamily member

• MW 21 kDa

• Synthesized by platelets and macrophages

• Induces differentiation in multiple cell types of various tissues

(13)

BMP

• Osteoinductive peptide family

• Belongs to the TGF-b superfamily

• MW 25-30 kDa

(14)

TÁMOP-4.1.2-08/1/A-2009-0011

FGF

• 23 members of the family

• Multiple functions on multiple cell types

• Human FGF-2 18 kDa non-glycosylated

polypeptide binds to heparin and heparan sulfate

(15)

PDGF

• Exists as a dimer

• Two chains MW 14-17 kDa each

• Synthesized by platelets, macrophages, endothelium, connective tissue

• Effects: Mitogenesis, angiogenesis, macrophage activation

(16)

TÁMOP-4.1.2-08/1/A-2009-0011

Delivery of growth factors

• Injected

• Diffused from scaffolds

• Released from gels

• Genetically engineered into cells

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